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塔依尔江.麦麦提,苏比奴尔.艾力,布威海丽且姆.阿巴拜科日,佐日古丽.伊斯马伊力,阿迪力.艾合麦提,伊拉木江.托合塔洪,马合木提.哈力克.2019.塔里木马鹿维生素D受体(VDR)基因结构 和功能的预测与分析.动物学杂志,54(5):693-700.
塔里木马鹿维生素D受体(VDR)基因结构 和功能的预测与分析
Prediction and Analysis of Structure and Function of Vitamin D Receptor (VDR) Gene in Tarim Red Deer
投稿时间:2019-04-09  修订日期:2019-09-02
DOI:10.13859/j.cjz.201905010
中文关键词:  塔里木马鹿  VDR基因  实时荧光定量PCR  生物信息学分析
英文关键词:Tarim red deer  VDR gene  Quantitative real-time PCR  Bioinformatics analysis
基金项目:国家自然科学基金项目(No. 31560600)
作者单位E-mail
塔依尔江.麦麦提 新疆大学生命科学与技术学院 1748863658@qq.com 
苏比奴尔.艾力 新疆大学生命科学与技术学院 1921675426@qq.com 
布威海丽且姆.阿巴拜科日 新疆大学生命科学与技术学院 346925145@qq.com 
佐日古丽.伊斯马伊力 新疆大学生命科学与技术学院 1985195899@qq.com 
阿迪力.艾合麦提 新疆大学生命科学与技术学院 1045954426@qq.com 
伊拉木江.托合塔洪 新疆大学生命科学与技术学院 ilhambhdr@sina.com 
马合木提.哈力克 新疆大学生命科学与技术学院 mahmuthalic@xju.edu.cn 
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中文摘要:
      为了分析塔里木马鹿(Cervus elaphus yarkandensis)维生素D受体(VDR)基因的结构和相关功能,本研究从前期研究获得的塔里木马鹿和天山马鹿(C. e. songaricus)皮肤组织转录组测序结果中,获得上调表达的塔里木马鹿VDR基因的序列,对塔里木马鹿VDR基因进行实时荧光定量PCR(qPCR)验证,利用相关软件进行同源性比对、系统进化树构建和生物信息学分析。实时荧光定量PCR结果显示,VDR基因在转录组测序的结果与qPCR的结果中表达趋势一致,均为上调。基于VDR基因同源性比对结果显示,塔里木马鹿与白尾鹿(Odocoileus virginianus,GenBank登录号XM_020889235.1)的遗传距离较近,同源性最高;与褐家鼠(Rattus norvegicus,GenBank登录号NM_017058.1)的遗传距离较远,同源性最低。系统进化树也证实了这个结果。生物信息学分析结果表明,塔里木马鹿VDR蛋白由20种氨基酸组成,分子质量为32.92 ku,理论等电点为5.73,不稳定系数为33.56,总平均亲水性为﹣0.298,脂溶系数94.95,无跨膜区,无信号肽,无O-糖基化位点,有1个N-糖基化位点,有15个磷酸化位点,最有可能位于内质网膜中,二级结构和三级结构主要由α-螺旋和无规则卷曲组成,有3个低复杂度区域,无保守结构区域。
英文摘要:
      This study was designed to analyze the structure and functions of vitamin D receptor (VDR) gene in Tarim red deer (Cervus elaphus yarkandensis). The expression of VDR gene was verified by quantitative real-time PCR (qPCR) based on transcriptome sequencing results. Homology comparison, phylogenetic tree construction and bioinformatics analysis were carried out using related software. The up-regulated expression trend of VDR gene in qPCR was consistent with that in transcriptome sequencing. The results of homology comparison and phylogenetic tree construction showed that the genetic distance between VDR gene of Tarim red deer and that of Odocoileus virginianus (GenBank accession number: XM_020889235.1) was the closest and the homology was the highest, while the genetic distance between VDR gene of Tarim red deer and that of Rattus norvegicus (GenBank accession number: NM_017058.1) was far and the homology was the lowest, which was confirmed by phylogenetic tree. Bioinformatics analysis showed that the Tarim red deer VDR protein was composed of 20 kinds of amino acids with molecular weight of 32.92 ku and its theoretical isoelectric point was 5.73. It was predicted that the instability index of VDR protein was 33.56, the lipid solubility index was 94.95, and its hydrophilic average coefficient was﹣0.298. VDR did not have transmembrane region, O-glycosylation site and signal peptide, but it had 1 N-glycosylation site and 15 phosphorylation sites, most likely located in the endoplasmic reticulum membrane. The secondary and tertiary structures of VDR protein were mainly comprised of α-helix and random coil. It had 3 low complexi regions and did not have conservative structure regions.
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