During the amphibian survey in Chishui Suoluo National Nature Reserve from 18 to 19 May, 2019, eight amphibian specimens were collected in Jinsha Ditch area (28°26′12″ N, 105°59′52″ E, altitude 465 m). The specimens were identified as Megophrys omeimontis, a new record of amphibians in Guizhou Province, China by morphological and molecular compare with the type locality specimen (Fig. 1 and 2, Table 2). The maximum likelihood phylogenetic tree constructed based on 16S rRNA gene fragments showed that the eight specimens were clustered with the M. omeimontis from type locality, and the genetic distance between our specimens and M. omeimontis from type locality was 0.04%, much lower than that between Megophrys species (Fig. 3, Table 3).