In January 2018, when investigating chiropteran diversity Wuyi Mountain in Jiangxi province, a male vespertilionid bat was captured by using harp trap. We identified this bat through morphological examination and phylogenetic inference. It is medium-sized bat with a forearm of 38.29 mm (Table 1) and the black-brown fur with white tip. Its tragus is triangular. Ears are nearly square with transverse ridges and connect in the forehead without a prominent projecting lobe (Fig. 1a). Besides, skull rises evenly from the rostral to the end, the vertex of skull is not prominent (Fig. 1c). The zygomatic arch is slender, while both sagittal and lambdoidal crests are weak. Its dental formula is 2.1.2.3/3.1.2.3 = 34 (Fig. 1b, c). According to its external, skull characteristics and phylogenetic evidences using partial Cyt b and ND1 gene, it is identified as Barbastella leucomelas which representing a new record species in Jiangxi Province, China.