| 王海梅,陈霞.2020.循环饥饿投喂对大鼠消化道嗜银细胞 形态及分布的影响.动物学杂志,55(2):222-228. |
| 循环饥饿投喂对大鼠消化道嗜银细胞 形态及分布的影响 |
| Effect of Cyclic Starvation on the Morphology and Distribution of Argyrophilic Cells in the Digestive Tract of Rats |
| 投稿时间：2019-10-09 修订日期：2020-03-16 |
| DOI：10.13859/j.cjz.202002011 |
| 中文关键词: 循环饥饿投喂 大鼠 嗜银细胞 消化道 形态 分布密度 |
| 英文关键词:Circulating hunger feeding Rat Argyrophil cells Digestive tract Morphology Distribution |
| 基金项目:哈尔滨师范大学硕士研究生创新科研项目（No. HSDSSCX2018-67） |
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| 中文摘要: |
| 为了研究循环饥饿投喂对大鼠（Rattus norvegicus）消化道内嗜银细胞的分布密度及形态功能的影响，本实验采用Grimelius银染法观察和测定循环饥饿投喂（饥饿1 d，投喂1 d，周期为14 d）期间大鼠消化道嗜银细胞形态功能及密度分布。结果表明，实验组（即循环饥饿投喂组）和对照组（正常喂食组）大鼠消化道嗜银细胞除食管外均有分布，形态上对照组嗜银细胞以椭圆形和锥体形为主，实验组嗜银细胞则主要以锥体形为主；两组大鼠消化道嗜银细胞的分布密度高峰都位于胃，密度低谷对照组位于空肠、盲肠、回肠和直肠，实验组位于空肠到直肠各段；实验组大鼠消化道嗜银细胞分布密度在贲门和幽门部极显著低于对照组（P < 0.01），结肠显著低于对照组（P < 0.05），说明循环饥饿投喂会明显改变消化道嗜银细胞的形态并降低细胞数量，这可能与内分泌细胞的功能改变有关。 |
| 英文摘要: |
| This experiment used Grimelius argyrophil staining to observe and measure the effect of cyclic starvation on the morphological function and distribution density of argyrophilic cells in the digestive tract of rats (Rattus norvegicus). Rats were starved for one day after being fed for one day for a period of 14 days. SPSS 25.0 was used to conduct one-way ANOVA on argyrophilic cells in each part of the digestive tract in the experimental group and the control group. Moreover, a statistical analysis was conducted on the distribution density of the argyrophilic cells in the same part of the digestive tract by using the independent sample T test. The results showed that the argyrophilic cells were distributed in all segments of the digestive tract except for the esophagus in both groups. Morphologically, the argyrophilic cells in control group were mainly ellipse and cone-shaped (Table 1), while those in the experimental group were mainly cone-shaped (Table 1). The distribution density of argyrophilic cells in gastrointestinal tract of the two groups was the highest in stomach (Table 2). The density of arginophilic cells in the stomach in the control group was 44.95 ± 7.87, and that in the experimental group was 42.35 ± 10.95. Low density of arginophilic cells was found in the jejunum, cecum, ileum and rectum of control group (Table 2) and that in the experimental group was found from jejunum to rectum (Table 2). The distribution density of argyrophilic cells in in the cardia and pylorus of the experimental group was very significantly lower than in the control group (P < 0.01), and that in the colon was significantly lower than that in the control group (P < 0.05). These results indicate that cyclic starvation will significantly change the morphology of argyrophilic cells and reduce the distribution density of the cells in the digestive tract, which may be related to the changes in the function of the endocrine cells. |
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