In July 2019, a bat was captured at Shihua Cave in Danzhou of Hainan Island and identified as Eonycteris spelaea by morphological measurements, skull characteristics and analysis on mitochondrial Cyt b gene. It is medium-sized bat with a forearm of 69.6 mm and without a claw on the second finger of the forelimb (Table 1). A short tail of 12.9 mm is prominent behind the femoral membrane and the bat has well-developed anal gland (Table 1 and Fig. 1). The greatest length of skull (GTL) is 35.2 mm and the rostral length (RL) is 11.4 mm (Table 1). The phylogenetic relationships of E. spelaea with its close species inferred from Cyt b gene sequences display that the Hainan bat and E. spelaea cluster a branch in high bootstrap value (Fig. 2). This is the first record of this species in Hainan Island. In the present study, morphological characters, the skull characters and the phylogenetic tree based on Cyt b gene sequences were represented detailedly. Comparisons were also performed between our findings and published data about the species recorded in other areas.