| 辛桂瑜,赵玲玉,贝永建,傅宏刚,卢玉莹,何相莲,黄彩诺,赖洁玲.2020.北部湾棱皮树蛙外周血细胞显微结构观察.动物学杂志,55(3):371-378. |
| 北部湾棱皮树蛙外周血细胞显微结构观察 |
| Microstructure of Peripheral Blood Cells in Theloderma corticale |
| 投稿时间：2019-11-04 修订日期：2020-04-29 |
| DOI：10.13859/j.cjz.202003011 |
| 中文关键词: 北部湾棱皮树蛙 血细胞 形态 数量 |
| 英文关键词:Theloderma corticale Hemocyte Morphology Quantity |
| 基金项目:环境保护部生物多样性保护专项，大学生创新训练项目（No. 201910606157，201710606036） |
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| 中文摘要: |
| 本文以人们极少关注的两栖动物北部湾棱皮树蛙（Theloderma corticale）为研究对象，采用瑞氏-姬姆萨混合染色法与血细胞计数法观察并统计了北部湾棱皮树蛙外周血细胞的形态特征和数量比例。结果显示，北部湾棱皮树蛙的外周血由红细胞、嗜中性粒细胞、嗜碱性粒细胞、嗜酸性粒细胞、淋巴细胞（包括大、小淋巴细胞）和单核细胞、血栓细胞7种细胞组成。其中，红细胞的数量最多，平均含量为2.43 × 105个/mm3，多呈椭圆形或卵圆形，少数呈泪滴形、梨形、逗号形、梭形，具椭圆或圆形核，核染色质密集，偶尔可观察到正在进行直接分裂或有丝分裂的红细胞。白细胞的数量比红细胞少，平均含量为1.74 × 104 个/mm3，其中，小淋巴细胞占40.02% ± 1.77%、大淋巴细胞占10.83% ± 2.53%、单核细胞占23.17% ± 3.16%、嗜中性粒细胞占10.08% ± 4.62%、嗜碱性粒细胞占9.25% ± 2.69%、嗜酸性粒细胞占6.72% ± 1.50%。血栓细胞呈纺锤形和梭形，常单独存在，或者呈圆形和不规则圆形，数个集合在一起。 |
| 英文摘要: |
| The morphological characteristics of hemocytes in peripheral blood were investigated in Theloderma corticale by the Wright-Giemsa staining method and manual counting of blood cells．The results showed that the peripheral hematocytes of the T. corticale were composed of erythrocytes, neutrophils, basophils and eosinophils, lymphocytes, monocytes, and thrombocytes (Fig. 1). Erythrocytes mostly were oval, elliptical and few were teardrop-shaped, pear-shaped, comma-shaped, spindle-shaped cells, and their mean density was 2.43 × 105 cells/mm3. Occasionally, erythrocytes undergoing direct division or mitosis were observed. The mean of leucocytes was 1.74 × 104 cells/mm3. The proportions of small lymphocytes, large lymphocyte, monocytes, neutrophils, basophils and eosinophils were 40.02% ± 1.77%, 10.83% ± 2.53%, 23.17% ± 3.16%, 10.08% ± 4.62%, 9.25% ± 2.69% and 6.72% ± 1.50%, respectively. Single thrombocyte was ovoid or spindle-like in shape and cluster thrombocyte was round and irregularly round in shape (Fig. 2 and Table 1). |
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