The high morbidity and mortality of captive Forest Musk Deer (Moschus berezovskii) due to the digestive tract diseases, especially the intestinal inflammatory diseases have been a major problem. Fecal detection is a basis for evaluating the health status of the digestive system of the wild animals and the clinical diagnosis of the intestinal inflammatory diseases. The fecal occult blood has a wide range of clinical diagnostic values in the diagnosis of gastrointestinal bleeding. In this study, diluted fresh Forest Musk Deer blood was utilized to explore the sensitivity ranges of three methods (pyramidon chemical method, o-toluidine chemical method and benzidine chemical method) for the determination of the blood concentration in the Forest Musk Deer feces. The results showed that the minimum sensitivity test concentration of the pyramidon method was 0.05 mg/L, more sensitive than that of o-toluidine method (0.4 mg/L) or benzidine method (100 mg/L). The positive rate of gastrointestinal bleeding revealed by the pyramidon method was significantly higher when compared to the other two methods, with the positive rate of pyramidon method 10.13%, o-toluidine chemical method of 2.56% and benzidine chemical method of 0 (P < 0.05). The operation of the pyramidon method was more convenient and rapid. Therefore, the detection of fecal occult blood in the Forest Musk Deer by pyramidon method is more accurate and convenient.