Effect of Temperature,pH and Salinity on Gill Na+-K+-ATPase Activity in Procambarus clarkii
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摘要:
采用钼蓝法测定克氏原螯虾(Procambarus clarkii)鳃Na+-K+-ATPase活性,探讨温度、pH、盐度3个环境因子在环境驯化和突变状态下对Na+-K+-ATPase活性的影响。实验结果表明,Na+-K+-ATPase的活性与环境因子密切相关。酶活性在温度、盐度驯化实验中都表现为正相关关系,不同pH驯化中则表现为中性pH活性最高。在突变状况下表现出明显的应激性,应激响应在2~8 h 之间,之后逐渐缓和,最终结果与驯化结果相同。
Abstract:
This paper aims to investigate the effect of environmental factors such as temperature,pH and salinity on the activity of gill Na+-K+-ATPase in freshwater crayfish,Procambarus clarkii under special environmental factors acclimation or their acute changes.In this research,phosphorus content—Molybdenum blue spectrophotometric method has been applied to determine the gill Na+-K+-ATPase activity in P.clarkii.The result indicates that activity of Na+-K+-ATPase varies in accordance to the fluctuations of environmental factors and acute effect of Na+-K+-ATPase was shown apparently in emergency conditions,which last for 2-8 h.The change became smooth afterwards and the finally activity was the same as the result in acclimate treatment.
