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邓时铭,田兴,曾春芳,刘丽,邹利,李传武,蒋国民.2020.湘华鲮外周血细胞形态特征及嗜中性粒细胞 吞噬与杀菌能力.动物学杂志,55(6):768-775.
湘华鲮外周血细胞形态特征及嗜中性粒细胞 吞噬与杀菌能力
Morphological Characters of Peripheral Blood Cells and Phagocytotic, Bactericidal Function of Neutrophils in Sinilabeo decorus tungting
投稿时间:2020-03-30  修订日期:2020-10-27
DOI:10.13859/j.cjz.202006010
中文关键词:  湘华鲮  外周血细胞  形态特征  吞噬与杀菌能力
英文关键词:Sinilabeo decorus tungting  Peripheral blood cells  Morphological character  Phagocytosis and bactericidal function
基金项目:湖南省科技重点研发计划项目(No. 2016NK2177)
作者单位E-mail
邓时铭 湖南省水产科学研究所 长沙 410153 dsmjgm@163.com 
田兴 湖南省水产科学研究所 长沙 410153 819569693@qq.com 
曾春芳 湖南省水产科学研究所 长沙 410153 33798074@qq.com 
刘丽 湖南省水产科学研究所 长沙 410153 460822360@qq.com 
邹利 湖南省水产科学研究所 长沙 410153 26440622@qq.com 
李传武 湖南省水产科学研究所 长沙 410153 湖南省水产原种场 长沙 410153 976222@qq.com 
蒋国民 湖南省水产科学研究所 长沙 410153 24048576@qq.com 
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中文摘要:
      采用血涂片、显微测量、血细胞计数及嗜中性粒细胞吞噬功能和氯化硝基四氮唑蓝还原试验方法,于光学显微镜下观察分析三种水体湘华鲮(Sinilabeo decorus tungting)外周血细胞形态结构、大小、种类及数量。结果表明,湘华鲮外周血细胞中有红细胞、血栓细胞、粒细胞、淋巴细胞和单核细胞,红细胞比例最高,达80%以上,白细胞中血栓细胞最多,占70% ~ 74%。大水面网箱养殖的湘华鲮外周血红细胞数量显著低于水泥池和土池养殖个体(0.01 < P < 0.05),而白细胞的数量、种类及各类白细胞所占比例较为稳定,不随养殖方式的变化而发生显著变化(P > 0.05),但水泥池湘华鲮嗜中性粒细胞的吞噬与杀菌能力较其他两种养殖方式显著下降(0.01 < P < 0.05)。因此,建议湘华鲮宜在水质良好的流水环境中养殖。
英文摘要:
      Sinilabeo decorus tungting, which belongs to Cyprinidae, Barbinae and Sinilabeo, is a large fish and endangered species distributed only in Hunan Province of China. In order to find a suitable culture environment, we studied the characteristics, immunofunctions and impact factors of peripheral hematocytes in S. d. tungting under different cultivation conditions (cage culture, cement-pond culture and soil-pond culture) (Table 1). The peripheral blood cells from 30 individuals were examined, classified, counted and photographed under the light microscope by blood smear, the diameters of various hematocytes and their nuclei in shortest and longest axes were measured using routine methods, and the immunofunctions of neutrophils were detected through phagocytosis test and nitroblue tetrazolium reduction test (NBT). The experimental data were analyzed with SPSS23.0, and the pictures were processed with photoshopCS. The results showed that the peripheral blood cells of S. d. tungting could be divided into five types as erythrocytes, thrombocytes, granulocytes, lymphocytes and monocytes, as shown in Fig. 1. The mean size of mature erythrocytes in length × width was 7.36 ± 0.58 μm × 5.16 ± 0.33 μm, while the largest blood cell in the peripheral blood cells was monocyte with a mean size of 11.93 ± 0.86 μm × 10.16 ± 0.76 μm (Table 2). The red blood cells consist of the largest proportion (over 80%) of peripheral blood cells, whereas the content of thrombocytes in low proportion white blood cells was as high as 70%﹣74% (Table 3). Among the three cultivation methods, the red blood cells number in cage culture was significantly lower than that in cement-pond culture or soil-pond culture (0.01 < P < 0.05); the number, type and ratio of white blood cells were relatively stable and did not change significantly with cultivation methods (P > 0.05, Table 3). In the phagocytosis test and nitroblue tetrazolium reduction test, results showed that the phagocytic percentage and phagocytosis index of neutrophils in cement-pond culture were significantly lower than in cage culture and soil-pond culture (0.01 < P < 0.05), and the positive rate of nitroblue tetrazolium reduction test was the lowest (P > 0.05). Therefore, the phagocytic and bactericidal ability of neutrophils in cement-pond culture was the worst (Table 4). According to the results of our experiment, S. d. tungting is suitable to live in a flowing, good quality water environment.
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