| 杨金权,胡雪莲,唐文乔,林弘都.2008.长江口邻近水域刀鲚的线粒体控制区序列变异与遗传多样性.动物学杂志,43(1):8-15. |
| 长江口邻近水域刀鲚的线粒体控制区序列变异与遗传多样性 |
| mtDNA Control Region Sequence Variation and Genetic Diversity of Coilia nasus in Yangtze River Estuary and Its Adjacent Waters |
| 投稿时间：2007-06-11 修订日期：2007-11-07 |
| DOI： |
| 中文关键词: 刀鲚 线粒体控制区 遗传多样性 长江口 |
| 英文关键词:Coilia nasus mtDNA control region Genetic diversity Yangtze River estuary |
| 基金项目:上海市科学技术委员会重大计划项目(No.04DZ19306);上海市农业委员会攻关项目[2003(1-4)];上海市重点学科建设项目(Y1101) |
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| 中文摘要: |
| 经克隆测序获得了采自长江口九段沙、钱塘江口、舟山等3个地点35尾刀鲚（Coilia nasus）的mtDNA控制区全序列,分析了控制区序列的变异和遗传结构。结果显示,长江口邻近水域刀鲚的mtDNA控制区序列具有长度多态性,全长为1214~1291bp,主要是在第358位点处有以"CTA TGT ATT ATA TTA CAT ATA TTA TGG TAT AGT ACA TA"38bp为单位的1~2次片段重复。种群遗传结构分析显示,长江口邻近水域刀鲚的平均单倍型多样性（h）为0.9983,3个群体的平均核苷酸多样性（π）为0.0262,表现出丰富的遗传多样性和较高的进化潜力。3个刀鲚群体间的分化指数FST仅为0.012~0.053,而基因交流值Nm却达9.90~40.62。群体间K2-P遗传距离与AMOVA分析结果也表明,长江口及毗邻地区的刀鲚没有发生地理分化。以线粒体DNA控制区全序列构建的NJ树揭示,3个群体的个体组成了2个谱系,但这2个谱系与地理分群并不相关。依据分子钟理论,推测这两个谱系的分化发生在更新世末期。 |
| 英文摘要: |
| We determined 35 complete mitochondrial control region sequences of Coilia nasus from 3 populations,which included Jiuduansha area(JJ)in Yangtze River estuary,Qiantangjiang River estuary(QT)and Zhoushan Island(ZS),to analyze their sequence variation and genetic structure.Sequence lengths of the control region in individuals of C.nasus were 1 214-1 291 bp,which revealed that the control region sequence of C.nasus had length polymorphism.Sequence length polymorphism caused mainly by one or two times of 38 bp of "CTA TGT ATT ATA TTA CAT ATA TTA TGG TAT AGT ACA TA" segment repeats.Genetic structure analysis showed a high level genetic diversity of C.nasus(h=0.998 3,π=0.026 2),which indicated that C.nasus had high evolutionary potential.The high level of Nm values(9.90-40.62)and very low F_ST values(0.012-0.053),the average K 2-P distances,as well as the AMOVA analysis all revealed that the C.nasus around Yangtze River estuary had no obviously geographical differentiation.However,the phylogenetic tree estimated by neighbor-joining(NJ)methods indicated that the individuals of C.nasus formed two monophyletic lineages,and the two lineages without correlation with geographical populations.Based on molecular clock theory,the two lineages might share a common ancestor approximately 35-47 thousand years ago. |
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