| 黄族豪,张姣,刘发,肖宜安,刘旻生,龙进.2008.两种竹鸡线粒体DNA的遗传变异.动物学杂志,43(1):21-25. |
| 两种竹鸡线粒体DNA的遗传变异 |
| Genetic Variation between Bambusicola thoracica and B.fytchii Based on Mitochondrial DNA |
| 投稿时间：2007-09-08 修订日期：2007-11-05 |
| DOI： |
| 中文关键词: 线粒体DNA控制区 灰胸竹鸡 棕胸竹鸡 遗传变异 |
| 英文关键词:Mitochondrial DNA D-loop Bambusicola thoracica B.fytchii Genetic variation |
| 基金项目:国家自然科学基金项目(No.30530130,30760036);江西省教育厅科技项目(赣教技字2007-323) |
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| 中文摘要: |
| 采用PCR和直接测序的方法测定灰胸竹鸡(Bambusicola thoracica)与棕胸竹鸡(B.fytchii)线粒体DNA(mtDNA)控制区1142bp的序列,分析二者间的遗传变异。两种竹鸡间共发现32个变异位点,其中20个转换,12个颠换。灰胸竹鸡mtDNA控制区的碱基含量T34.26%、C25.98%、A24.84%和G14.96%,棕胸竹鸡的分别是T34.47%、C25.60%、A25.03%和G14.93%。t-检验分析显示,两种竹鸡mtDNA控制区的T和C含量差异显著。在系统发生树上,两种竹鸡在系统发生树各聚成一支,支持率达到100%。两种竹鸡间的遗传距离是0.0396。根据分子钟计算,它们大约在200万年前分歧进化。推测它们的物种形成主要受更新世第二次寒冷期的影响。 |
| 英文摘要: |
| Polymerase chain reaction and direct sequencing methods were used to analyze the genetic variation between Bambusicola thoracica and B.fytchii.A total of 1 142 nucleotides of the mitochondrial DNA control region were sequenced.There are 32 variable positions,with 20 transitions and 10 transversions.The nucleotide composition of B.thoracica mtDNA D-loop was:34.26% T,25.98% C,24.84% A and 14.96% G.That of B.fytchii was:34.47% T,25.60% C,25.03% A and 14.93% G.There were very significant differences in T and C nucleotide composition between the two bamboo partridges.The phylogenetic tree grouped two bamboo partridges into two deeply divergent clusters(supported by BP=100%).Genetic distance was 0.039 6 between the two partridges.Calibrated rates of molecular evolution suggested that they could have speciated 2.00 million years ago,affected by the Pleistocene second frigid. |
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