| 刘海岩,葛敬岩,陈芳芳,台桂香,柳忠辉.2008.激活素受体相互作用蛋白1在小鼠组织中的表达与分布.动物学杂志,43(1):26-30. |
| 激活素受体相互作用蛋白1在小鼠组织中的表达与分布 |
| Messenger RNA Expression and Protein Distribution of Activin Receptor-interacting Protein 1 in Mouse Tissues |
| 投稿时间：2007-05-14 修订日期：2007-11-14 |
| DOI： |
| 中文关键词: ARIP1 mRNA 表达 分布 脑 |
| 英文关键词:ARIP1 Messenger RNA Expression Distribution Brain |
| 基金项目:国家自然科学基金项目(No.30440044,30170298,30170478) |
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| 中文摘要: |
| 激活素具有调节激素分泌以及神经保护等多种作用,最近在小鼠脑内发现的激活素受体相互作用蛋白1(ARIP1)具有介导激活素信号传导作用,但有关ARIP1的分布情况仍然不清楚。本研究采用RT-PCR及免疫组织化学染色分析ARIP1在脑及脑外的表达与分布情况。RT-PCR检测发现ARIP1 mRNA不仅在大脑、小脑表达,在垂体、肾上腺以及睾丸也有明显表达。免疫组化染色显示大脑、小脑、垂体、肾上腺和睾丸均有不同程度的ARIP1免疫染色反应,小脑中浦肯野细胞着色明显,大脑主要是海马和下丘脑,在神经垂体、腺垂体的嗜碱细胞以及肾上腺网状带、球状带、束状带中均有表达,睾丸间质细胞也可见ARIP1成熟蛋白表达。结果提示,ARIP1不仅参与脑神经细胞的信号传导调节,也可能参与神经内分泌腺的功能调节。 |
| 英文摘要: |
| Activin plays an essential role in the neuroprotection as a nerve survival factor.Activin receptor-interacting protein1(ARIP1)has been recently discovered in mouse brain as a regulator of activin signal transduction,however,its distribution pattern is not well characterized.In this study,the expression of ARIP1 mRNA and localization of mature ARIP1 protein in mouse tissues were examined by reverse transcription-polymerase chain reaction(RT-PCR)and immunohistochemistry,respectively.ARIP1 mRNA expression was detectable in cerebrum,cerebellum,extra-brain tissues such as pituitary,adrenal gland and testis.Immunohistochemical analysis showed that ARIP1-like immunoreactivity protein was mainly localized in hypothalamus and hippocampus of the cerebrum,Purkinje cells of the cerebellum,neurohypophysis and basophilic cells of adenohypophysis of the pituitary,adrenal cortex of the suprarenal gland,and Leydig cells of the testis.These data suggest that ARIP1 may participate in not only the regulation of activin signal transduction of nerve cells,but also the regulation of function in neural endocrine gland. |
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