| 刘岑杰,刘欣,吴毓,马飞,王继红,李庆伟.2008.日本七鳃鳗类淋巴细胞的分离及细胞学特征.动物学杂志,43(1):82-87. |
| 日本七鳃鳗类淋巴细胞的分离及细胞学特征 |
| Separation and Cytological Character of Peripheral Blood Lymphocytes in Japanese Lamprey |
| 投稿时间：2007-06-08 修订日期：2007-11-13 |
| DOI： |
| 中文关键词: 日本七鳃鳗 类淋巴细胞 流式细胞分选 细胞异质性 |
| 英文关键词:Lampetra japonica Lymphocyte-like cells Cytometery Cell heterogeneity |
| 基金项目:国家重点基础研究发展计划项目(No.2007CB815802);国家高技术研究发展计划项目(No.2007AA09Z400) |
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| 中文摘要: |
| 为分离纯化并鉴定日本七鳃鳗(Lampetra japonica)血液中的类淋巴细胞,采用Ficoll密度梯度离心法分离出单个核细胞层,并得到分离单个核细胞层的最佳分离液比重为1.092。利用流式细胞仪对分离到的单个核细胞层细胞进行分选,根据细胞的前向光及侧向光散射特征成功分选出类淋巴细胞,分选效率为95.68%,每毫升外周血可分离纯化得到类淋巴细胞2.4×106个。通过透射电镜观察七鳃鳗类淋巴细胞,细胞为圆形或椭圆形,细胞表面有突起、无微绒毛,胞质内含有板状嵴线粒体、粗面内质网、游离核糖体和液泡等。淋巴细胞异质性实验结果表明,日本七鳃鳗血液白细胞中尚未发现T淋巴细胞、B淋巴细胞的分化。 |
| 英文摘要: |
| To isolate and purify peripheral blood lymphocyte-like cells from the arctic lamprey,Lampetra japonica,centrifugation on Ficoll-Paque was performed and a uniform population of leucocytes containing lymphocyte-like cells with the mixture of Ficoll-Urografin solution at proportion of 1.092 was yielded.With the FSC and SSC character of the cells on flow cytometer,lymphocyte-like cells were separated successfully,and the purity was 95.68%.About 2.4×106 lymphocyte-like cells could be purified from 1 ml peripheral blood.Under TEM,lymphocyte-like cells were round or ellipse in shape without microvilla.There were mitochondria with cristae,rough endoplasmic reticulum,dissociated ribosomes and vacuoles in the cytoplasm.Lymphocyte differentiation analysis revealed that there was no evident differentiation of T and B lymphocytes in the leucocytes of L.japonica. |
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