Sperm shape of 7 species(subspecies)of the genus Bufo was observed by light and scanning electron microscope,and the size of spermatozoa were also measured.The results show that the sperm from the 7 species(subspecies)are basically similar in shape:the sperm are composed of head,mid-piece and tail;the head is thin,long and slightly curved;the mid-piece is relatively evident,and the tail is long and winding with an undulating membrane.The shape of spermatozoa from genus Bufo differs remarkably from that of other families of Anura,but is more similar to that of Urodela.The spermatozoa differ in morphology and size among different families of Amphibia,and the diversity of sperm morphology of Amphibia might be related to their different reproductive modes.