The nutritional composition in muscle of two Bottlenose Dolphins(Tursiops truncatus)was determined.Samples were taken from thorax,back,belly and tail.The results showed that the difference of crude nutritional composition was very little except for the content of lipids,which was higher in belly and tail than in other place.Content of essential amino acids was much higher in back and thorax than in other position,but ratio of E/T is surprisingly similar.Glu was highest and Csy was lowest among 18 amino acids.There has not significant difference for fatty acids composition in different samples.Typical fatty acids were found in the major part of both specimens,such as palmitic(16:0),palm oleic acid(16:1),stearic(18:0),oleic(18:1),EPA(20:5 ω-3)and DHA(22:6 ω-3).SAF was very close to MUFA in amount,and higher than PUFA.The ratio of polyenes ω-3/ω-6 was normally lower than other marine organisms.Muscle of dolphins was rich in Ca,Fe and trace metals such as Zn,Cu,Mn,Se.