Light microscopy,scanning electron microscopy and microvascular casting technique were used to observe the renal tissue structure and microvasculature in the Panthera pardus.The results showed that the thickness ratio between substantia corticalis and substantia medullaris of the renal was 1∶(1.5-3.0).The proximal tubular epithelium was simple cuboidal,with strong eosinophilic cytoplasm.The boundary between big and closely-arranged epithelial cells was not clear.Brush border was observed inside proximal tubule but not distal tubule.The distal tubular epithelial cells were low columnar cells with weak eosinophilic cytoplasm.The nucleus was located in the middle of the cell.The epithelial cells of collecting tubule with weak eosinophilic staining were closely arranged and the lumen of collecting tubule was big.The renal glomus was spheroidal in shape,and its diameter was 99-142 μm.The diameter of afferent glomerular arteriole was 13-15 μm.The diameter of efferent glomerular arteriole was 9.5-11.5 μm.