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魏锦瑜,高欣,杨仙玉.2008.利用铁葡聚糖分离纤毛虫接合对的方法.动物学杂志,43(2):77-80.
利用铁葡聚糖分离纤毛虫接合对的方法
Introducing a Method for Separating Ciliate Conjugating Pairs by Magnetic Iron-dextran Particles
投稿时间:2007-09-29  修订日期:2007-11-10
DOI:
中文关键词:  纤毛虫  接合对  铁葡聚糖颗粒
英文关键词:Ciliate  Conjugating pairs  Iron-dextran particles
基金项目:国家自然科学基金项目(No.30670297)
作者单位
魏锦瑜 浙江林学院林业与生物技术学院 临安 311300 
高欣 浙江林学院林业与生物技术学院 临安 311300 
杨仙玉 浙江林学院林业与生物技术学院 临安 311300 
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中文摘要:
      介绍一种制备微小铁葡聚糖颗粒以及利用微小铁葡聚糖颗粒分离纤毛虫接合对的方法,通过本方法可以获得接合率达95%以上,且发育基本同步的实验材料。
英文摘要:
      In this report,methods for preparing magnetic iron-dextran particles and separating highly synchronized (95%) ciliate conjugating pairs are introduced.When iron-dextran particles are added to the mixtures of complementary mating types of ciliates,single cells and loose conjugating pairs form food vacuoles containing the iron-dextran particles while absent from the tight mating pairs.By applying magnetic force,single cells and loose mating pairs can be removed from the tight conjugating pairs.This method has several advantages: simplicity,speediness,high quality,universality and practicality.No negative effects have been found when perform the experiments by this method.
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