| 谢静,王政昆,张武先,朱莉萍.2008.冷暴露对中缅树鼩褐色脂肪组织中解偶联蛋白1含量的影响.动物学杂志,43(4):34-40. |
| 冷暴露对中缅树鼩褐色脂肪组织中解偶联蛋白1含量的影响 |
| The Effects of Cold Exposure on the Content of Uncoupling Protein of BAT in Tupaia belangeri |
| 投稿时间：2007-12-26 修订日期：2008-04-28 |
| DOI： |
| 中文关键词: 中缅树鼩 冷暴露 解偶联蛋白-1 酶联免疫 |
| 英文关键词:Tupaia belangeri Cold acclimation Uncoupling protein-1 Enzyme-linked immunosorbent assay |
| 基金项目:国家自然科学基金项目(No.30560026);云南省科技强省研究重点项目(No.2007C000Z1) |
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| 中文摘要: |
| 自备抗血清采用酶联免疫法测定了中缅树鼩(Tupaiabelangeri)在(5±1)℃冷暴露0d、7d、14d、21d、28d时,褐色脂肪组织(BAT)中解偶联蛋白1(UCP1)的含量。结果表明,随着冷暴露时间的延长,中缅树鼩的体重、褐色脂肪组织重量均表现出了增加的趋势,BAT线粒体总蛋白和UCP1的含量也呈增加的趋势,其中UCP1的含量在28d时达到极显著水平,比对照组增加了55.9%。说明冷暴露能够诱导中缅树鼩UCP1表达增加,从而使其适应性产热增加。 |
| 英文摘要: |
| Uncoupling proteins(UCPs) increase proton leakage across the inner mitochondrial membrane.UCP1 in brown adipose tissue dissipates proton motive force as heat.This mechanism of nonshivering thermogenesis is considered as a monophyletic trait of endothermic placental mammals and provided a crucial advantage for life in the cold.Thereby,UCP1 is thus the only protein capable of mediating cold-acclimation-recruited adaptive adrenergic nonshivering thermogenesis.In this study,the content of UCP1 in BAT in Tree Shrews(Tupaia belangeri) was measured during cold exposure for 0 day,7 days,21 days and 28 days by enzyme-linked immunosorbent assay.The body weight,BAT weight,mitochondrial protein contents and UCP1 contents greatly increased with the cold exposure.The UCP1 content increased by 55.9%(P<0.01) significantly after 28 days cold acclimation.The changes in BAT thermogenic properties are important for the survival under cold stress in T.belangeri. |
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