| 李华,孙虎山,王宜艳,李霞.2008.扁玉螺体表和消化系统甲硫氨酸脑啡肽免疫组织化学定位.动物学杂志,43(4):72-76. |
| 扁玉螺体表和消化系统甲硫氨酸脑啡肽免疫组织化学定位 |
| Immunohistochemical Localization of Methionine-enkephalin in the Body Surface and Digestive System of Neverita didyma |
| 投稿时间：2008-01-10 修订日期：2008-04-23 |
| DOI： |
| 中文关键词: 甲硫氨酸脑啡肽 免疫组织化学 消化系统 体表 扁玉螺 |
| 英文关键词:Methionine-enkephalin Immunohistochemistry Digestive system Body surface Neverita didyma |
| 基金项目:国家“973”计划项目(No.G1999012005) |
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| 中文摘要: |
| 采用免疫组织化学streptavidin-biotincomplex(S-ABC)法对甲硫氨酸脑啡肽(methionine-enkephalin,M-ENK)在扁玉螺(Neveritadidyma)体表、消化系统各器官中的分布进行了研究。结果表明,扁玉螺的足上皮细胞、外套膜的内外上皮细胞以及食道、胃、肠道的黏膜上皮细胞均呈M-ENK阳性反应,且消化道中的阳性反应多集中于上皮细胞游离端;在食道腺的腺上皮中也有少量阳性细胞分布;肝是M-ENK阳性细胞分布较多的器官,主要分布在肝小叶中腺细胞边缘游离端。M-ENK在扁玉螺体表和消化系统各器官均有分布,且分布密度有所不同,可能与各部位的功能有关。 |
| 英文摘要: |
| The methionine-enkephalin(M-ENK)in the body surface and digestive system of Neverita didyma was analyzed by the immunohistochemical S-ABC(strept avidin-biotin complex) staining.The results showed that M-ENK positive cells existed widely in the epithelium of foot,inner and outer epithelia of mantle,mucous membrane epithelium of oesophagus,stomach and intestine.Besides,the positive immuno-reaction was centralized in the dissociative sides of alimentary canal epithelium.A small quantity of positive cells was observed in the esophageal gland,too.A great number of positive cells were observed in the liver,mostly in the dissociative sides of the gland cells.It can be concluded that M-ENK is abundantly distributed in the epidermis of the body surface and digestive system in N.didyma,and that different densities of M-ENK in these organs may be referable to their different functions. |
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