| 陆宏达,朱光来,张连义,卫胜昌,倪云超.2008.异育银鲫上皮瘤中黏液细胞类型、分布和分泌方式.动物学杂志,43(4):85-91. |
| 异育银鲫上皮瘤中黏液细胞类型、分布和分泌方式 |
| Type,Distribution and Secreting Activity of Mucous Cells in the Epidermal Tumor in Allogynogenetic Crucian Carp |
| 投稿时间：2008-02-04 修订日期：2008-04-29 |
| DOI： |
| 中文关键词: 异育银鲫 上皮瘤 黏液细胞 类型 分布 分泌方式 |
| 英文关键词:Carassius auratus gibelio ♀ × Cyprinus carpio var.singuonensis ♂ Epidermal tumor Mucous cells Type Distribution Secretion |
| 基金项目:上海市农委重点攻关项目[沪农科攻字(2006)第6-6号];上海市重点学科建设项目(Y1101) |
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| 中文摘要: |
| 采用苏木精-伊红(H.E)和阿利新蓝-过碘酸雪夫试剂(AB-PAS,AB染液pH2.6)染色方法对异育银鲫(Carassiusauratusgibelio♀×Cyprinuscarpiovar.singuonensis♂)上皮瘤组织中黏液细胞的类型、分布、大小和分泌方式进行了研究。根据AB-PAS的染色结果,上皮瘤组织中黏液细胞分为4种类型:染成红色的Ⅰ型黏液细胞,只含有PAS阳性的中性黏多糖;染成蓝色的Ⅱ型黏液细胞,只含有AB阳性的酸性黏多糖;染成紫红色的Ⅲ型黏液细胞,主要含有PAS阳性的中性黏多糖,同时含有少量AB阳性的酸性黏多糖;染成蓝紫色的Ⅳ型黏液细胞,主要含有AB阳性的酸性黏多糖,同时含有少量PAS阳性的中性黏多糖。Ⅰ型和Ⅱ型黏液细胞的数量相对较少,以分散状态分布于上皮瘤组织的上皮细胞之间,以其整个细胞破坏的全浆分泌方式进行分泌。Ⅲ型和Ⅳ型黏液细胞的数量较多,成团的形式分布在上皮瘤组织小叶中央,同样以整个细胞崩解死亡而消失的全浆分泌方式进行分泌。当患病鱼受到刺激时,Ⅲ型和Ⅳ型黏液细胞分泌十分旺盛,分泌物排出后覆盖在上皮瘤组织表面,形成白色的黏液层,黏液细胞崩解死亡消失后在上皮瘤组织中留下许多空洞。患病鱼黏液细胞中,Ⅲ型黏液细胞最大,大小为(19.02±3.21)μm×(14.42±2.69)μm;其次是Ⅳ型黏液细胞,大小为(15.97±3.31)μm×(12.96±2.81)μm;Ⅱ型黏液细胞较小,大小为(13.35±3.81)μm×(11.05±3.00)μm;Ⅰ型黏液细胞最小,大小为(11.73±2.45)μm×(7.71±0.97)μm。各类型黏液细胞的长轴之间及短轴之间均存在显著性差异(P<0.05)。 |
| 英文摘要: |
| The type,distribution,size and secreting activity of mucous cells stained with H.E and AB-PAS(AB pH =2.6) in the epidermal tumor in Allogynogenetic Crucian Carp(Carassius auratus gibelio ♀ × Cyprinus carpio var.singuonensis ♂) have been studied under light microscope.Based on the observation after AB-PAS stain,the mucous cells in the epidermal tumor were divided into four types: Type Ⅰ which was only PAS positive was in red color,containing neutral mucoitin;Type Ⅱ which was only AB positive was in blue color, containing acid mucoitin;Type Ⅲ which was strong PAS positive and slight AB positive was in purple reddish color,containing mainly neutral mucoitin and slightly acid mucoitin: Type Ⅳ which was strong AB positive and slight PAS positive was in blue purple color,containing mainly acid mucoitin and slightly neutral mucoitin.The type Ⅰ and Ⅱ mucous cells were relatively fewer,and individually distributed among the epithelial cells of epidermal tumor.The secreting method of type Ⅰ and Ⅱ mucous cells was holocrine secretion.The type Ⅲ and Ⅳ mucous cell mainly distributed in cluster in the centre of leaf-like folds of tumor tissue.The secreting method of type Ⅲ and Ⅳ mucous cells was also holocrine secretion which gradually died and disappeared after secreting.When the diseased fish were stressed,the secreting activity of type Ⅲ and Ⅳ mucous cells was more exuberant.The surface of epidermal tumor was covered with mucus which formed white mucus layer.The empty spaces and holes were left after the lyses of mucous cells.Type Ⅲ mucous cell was largest in size(19.02±3.21 μm×14.42±2.69 μm);Type Ⅳ mucous cell was second(15.97±3.31 μm×12.96±2.81 μm);Type Ⅱ mucous cell was third(13.35±3.81 μm×11.05±3.00 μm);Type Ⅰ mucous cell was smallest(11.73±2.45 μm×7.71±0.97 μm).There was significant difference in sizes between different types of mucous cells. |
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