| 朱道玉.2008.中华鳖眼睛发育的形态及组织学观察.动物学杂志,43(4):97-101. |
| 中华鳖眼睛发育的形态及组织学观察 |
| Morphological and Histological Observation on the Eye Development of Trionyx sinensis |
| 投稿时间：2007-12-20 修订日期：2008-04-23 |
| DOI： |
| 中文关键词: 中华鳖 眼睛 发育 |
| 英文关键词:Trionyx sinensis Eye Development |
| 基金项目:菏泽学院科研基金项目(No.XY05SW03) |
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| 中文摘要: |
| 在孵化基质沙粒径为0.3~0.6mm、孵化温度为(33.0±0.5)℃、孵化基质的湿度为7%~10%、相对湿度为70%~85%的条件下孵化中华鳖(Trionyxsinensis)卵,孵化周期35~36d。破壳取不同发育时期的胚胎并制作切片,观察眼睛发育的形态学和组织学特征。孵化第4d头部两侧出现眼泡的突起;第6d眼睛开始出现色素,第14d色素由褐色变为黑色;第7d瞳孔出现,透过瞳孔可见晶状体;虹膜于第14d出现,第18、19d瞳孔周围呈放射状;巩膜突自第19d出现,第21d增至最多,第23d消失;上、下眼睑分别在第19d和22d出现,第32d眼睑可覆盖瞳孔,眼睛形态与成体眼睛相似。表皮外胚层于第3d形成角膜原基和晶体泡,第32d角膜发育完成;第34d晶状体发育完成;神经外胚层于44~48h由前脑的两侧分化形成视泡,第3d由视泡分化形成视杯,并逐步分化形成视网膜;第23d视网膜的八层结构基本形成;第34d视网膜发育完成。 |
| 英文摘要: |
| Eggs of Trionyx sinensis were incubated in arenaceous substrate with the diameter of sand granule 0.3-0.6 mm and the humidity 7%-10%.The temperature incubated was 33.0±0.5℃ and the relative humidity was 70%-85%.The incubated period was 35-36 d.Embryos of different developmental stage were taken out through breaking up eggshells.Slices were made in order to observe morphological and histological characteristics of the eye development of T.sinensis.Protuberances of eye vesicle appeared on tow sides of the head on 4th day after incubated.The pigment began to appear in eyes on the 6th day.On the 14th day,brown pigment became black.On the 7th day,the pupil appeared.The crystalline lens was visible through the pupil.The iris appeared on the 14th day.It became actinomorphic around the pupil in the 18th and 19th day.The scleral stalk was appeared on the 19th day,increased to the maximum on the 21st day and disappeared on the 23rd day.The upper eyelid and the lower eyelid appeared on the 19th day and the 22nd day respectively.The eyelid was able to cover the pupil on 32nd day,and the eye was similar to mature eye in the form.The cutis ectoderm formed the cornea primordial and the lens vesicle after the 3rd day.After the 32nd day,the cornea developed completely.The crystalline lens developed completely after the 34th day.The nerve ectoderm differentiated and formed the optic vesicle on tow sides of the forebrain from the 44th to 48th hour.The optic cup,which was differentiated from the optic vesicle,gradually differentiated and formed the retina.8-stratum structure of the retina was basically formed after the 23rd day.The retina developed completely after the 34th day. |
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