刘秀霞,梁旭方,丁雪芬,王琳,林群,李光照,沈丹.2008.微囊藻毒素对尼罗罗非鱼原代肝细胞致毒机理的探讨.动物学杂志,43(5):25-30. |
微囊藻毒素对尼罗罗非鱼原代肝细胞致毒机理的探讨 |
Microcystin-LR Toxicity on Primary Cultured Hepatocyte from Oreochromis niloticus |
投稿时间:2007-12-22 修订日期:2008-07-02 |
DOI: |
中文关键词: 微囊藻毒素(MC-LR) 原代肝细胞 致毒机理 尼罗罗非鱼 |
英文关键词:Microcystin-LR(MC-LR) Primary cultured hepatocytes Toxicity mechanism Nile Tilapia(Oreochromis niloticus) |
基金项目:国家自然科学基金项目(No.30670367);广东省科技计划项目(No.2007B02070100,No.2005B20301005);教育部留学回国人员科研启动基金项目 |
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中文摘要: |
采用离体细胞培养诱导方法,研究微囊藻毒素-LR(microcystin-LR,MC-LR)对尼罗罗非鱼(Oreochromis niloticus)原代肝细胞的毒性效应。尼罗罗非鱼原代肝细胞经10、50、150、500μg/L MC-LR体外诱导24h后,单细胞微量凝胶电泳(SCGE)检测显示,与对照组相比处理组出现明显的彗星拖尾现象,说明MC-LR可引起尼罗罗非鱼肝细胞DNA的损伤,并随着剂量的增加,DNA的损伤程度增大。PI/Annexin V双染色流式细胞仪(FCM)检测表明MC-LR能明显引起肝细胞凋亡,与SCGE结果一致,且DNA损伤程度越大,细胞早期凋亡率越高,呈现明显的时间、剂量依赖性。本研究为进一步从分子、细胞水平阐明MC-LR的毒性以及致毒机理提供重要的理论依据。 |
英文摘要: |
The isolated hepatocytes of Nile Tilapia(Oreochromis niloticus)were exposed to MC-LR to study the mechanism of microcystin-LR(MC-LR)toxicity.After hepatocytes were exposed to 10,50,150,500 μg/L MC-LR for 24 h respectively,the single cell gel electrophoresis assay(SCGE)was used to investigate the effects on DNA.Induction of DNA strand break in all MC-LR exposed groups was significantly higher than that of the control group.Treatment time-and dose-dependent DNA migration caused by MC-LR and was observed.Flow cytometry(FCM)analysis indicated that the percentage of hepatocyte apoptosis increased in a treatment time-and dose-dependent manner.These results provide first experimental evidence showing the mechanism of MC-LR toxicity at cellular and molecular levels. |
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