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刘军须,蔡月花,张敬各,刘福英.2008.雄性不育大鼠近交系MIJ的建立及其遗传特征观察.动物学杂志,43(5):37-44.
雄性不育大鼠近交系MIJ的建立及其遗传特征观察
Establishment and Genetic Characteristics of Rat Inbred Strain MIJ with Spontaneous Male Infertility
投稿时间:2007-12-30  修订日期:2008-07-10
DOI:
中文关键词:  雄性不育  近交系  大鼠  遗传特征  小睾丸
英文关键词:Male infertility  Inbred strain  Rat  Genetic characteristic  Small testis
基金项目:河北省科技攻关计划项目(No.07275503);河北省科技条件建设项目(No.2008090008)
作者单位
刘军须 河北医科大学实验动物学部河北省实验动物重点实验室 石家庄 050017 
蔡月花 河北医科大学实验动物学部河北省实验动物重点实验室 石家庄 050017 
张敬各 河北医科大学法医学系 石家庄 050017 
刘福英 河北医科大学实验动物学部河北省实验动物重点实验室 石家庄 050017 
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中文摘要:
      以发现的雄性不育大鼠的正常表型同胞鼠为种鼠,通过全同胞近亲交配方式,采用选优法成功培育出自发雄性不育大鼠近交系MIJ。同系异体皮肤移植验证其遗传纯合度,观察其表型特征,并通过遗传测交试验测定雄性不育性状的遗传方式。结果表明,MIJ种群符合近交系标准;雄性不育大鼠呈睾丸下降延迟、睾丸及附睾发育迟缓、精子数量少及精子生成障碍;近交系内其他个体表型正常。不育性状呈隐性遗传方式,由常染色体上单一隐性基因控制。该雄性不育大鼠可作为人类男性不育研究的动物模型。
英文摘要:
      The normal sibling rats of the infertile male were selected for brother-sister breeding.The stocks of every filial generation whose male siblings were infertile were inbred with their brothers or sisters by optimum seeking method.After 20 generations,a rat inbred strain of spontaneous male infertility was established,and was named MIJ.The genetic homozygosity was analyzed by skin grafting.The phenotypes of MIJ were observed,and the mode of infertility inheritance was studied by test cross experiments.The results demonstrated that MIJ rats had reached the norm of inbred strain,and the infertile male rats showed the phenotypes of delayed testicular descent,testicular and epididymis hypoevolutism,oligozoospermia and hypospermatogenesis.The phenotypes of the other rats in the inbred strain were normal.The infertility mutation was inherited in a recessive mode,and was controlled by a monogenic recessive gene in euchromosome.The infertile male rats could serve as a useful model for the study of human infertility.
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