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3种蝙蝠听力相关基因EYA4(eyes absent 4)开放阅读框序列的克隆与分析
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华东师范大学紫江学者资助项目


Cloning and Analysis of Open Reading Frame of Hearing-correlated Gene EYA4 (Eyes Absent 4) in Three Species of Bats
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    摘要:

    EYA4基因是与果蝇(Drosophila melanogaster)的无眼基因eya同源的4个脊椎动物转录激活因子eya基因家族(EYA1-4)的一员。相关的研究表明EYA4基因与非综合征听觉障碍有关。蝙蝠特化的听觉系统在回声定位生理过程中负责回声信息的接收和处理,并起到重要作用。利用RT-PCR技术,通过克隆测序得到3种蝙蝠(大足鼠耳蝠Myotis ricketti,黑髯墓蝠Taphozous melanopogon,棕果蝠Rousettusles chenaulti)EYA4基因的开放阅读框序列。通过比对分析,发现这3种蝙蝠均没有第五外显子,而在第十一外显子与第十二外显子之间有一个其他哺乳动物所没有的外显子exon b。此外,实验结果还显示,在棕果蝠中没有发现其他已知哺乳动物EYA4基因的第二十外显子;在黑髯墓蝠EYA4基因中缺少第十九外显子,而且与其他哺乳动物相比,黑髯墓蝠EYA4基因具有较短的第十六外显子,即缺少了30个碱基;大足鼠耳蝠与非蝙蝠哺乳动物一样存在第十九和第二十外显子的可变剪接模式,且在第二与第三外显子中间存在外显子exon a。

    Abstract:

    EYA4,homologous to eyes absent(eya)gene of Drosophila,is a member of the four vertebrate transcriptional activators of eya gene family.Previous studies have indicated that EYA4 gene is interrelated with sensorineural hearing loss.The specialized auditory system of bat that receives and processes echo information has an important role in echolocation.Using the RT-PCR method,the open reading frame(ORF)of EYA4 gene was cloned and sequenced in three species of bats(Myotis ricketti,Taphozous melanopogon,and Rousettus leschenaulti).Sequence alignment indicates that the EYA4 gene of all these three bats does not have exon 5 but has a unique exon b between exon 11 and exon 12.Compared to the EYA4 gene of other mammals,R.leschenaulti does not have exon 20 and T.melanopogon does not have exon 19.Moreover,T.melanopogon has a 30 bp deleted exon 16.M.ricketti has variable splicing of exon 19,exon 20 and exon a between exon 2 and exon 3.

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王力,王锦红,何贝贝,周莹莹,叶绍辉,李钢.2008.3种蝙蝠听力相关基因EYA4(eyes absent 4)开放阅读框序列的克隆与分析.动物学杂志,43(5):62-68.

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  • 收稿日期:2008-02-26
  • 最后修改日期:2008-07-10
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