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利用错配碱基产生酶切位点快速检测禽类Mx蛋白基因单核苷酸改变
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北京市自然科学基金项目(No.5051004)


An Effective Method for Detecting SNP Using Mismatched Bases to Create Specific Restriction Enzyme Cutting Sites
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    摘要:

    该方法针对无合适酶切位点的检测位点设计引物,应用于禽类Mx蛋白基因631位氨基酸位点的突变检测,利用错配碱基产生内切酶识别的酶切位点检测SNP,尝试一种操作简便结果可靠的检测单核苷酸多态性并能快速判定631位点氨基酸的方法。实验结果表明,利用这种方法产生的酶切位点能有效地对631位氨基酸位点进行分型,可以快速检测SNP。

    Abstract:

    In order to find a simple and effective way to detect SNP,a method for creation of restriction enzyme cutting sites was developed by using mismatched primers.This method can be used to detect SNP effectively and rapidly.

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尹春光,李善刚,路国彬,杜立新.2008.利用错配碱基产生酶切位点快速检测禽类Mx蛋白基因单核苷酸改变.动物学杂志,43(5):80-85.

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  • 收稿日期:2008-03-28
  • 最后修改日期:2008-07-02
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