| 王庆恒,杜宇宏,林斯里,杜晓东,邓岳文.2008.裸体方格星虫染色体组型分析.动物学杂志,43(5):95-98. |
| 裸体方格星虫染色体组型分析 |
| Karyotype Analysis of Sipunculus nudus |
| 投稿时间：2007-12-09 修订日期：2008-06-26 |
| DOI： |
| 中文关键词: 裸体方格星虫 染色体 组型分析 |
| 英文关键词:Sipunculus nudus Chromosome Karyotype |
| 基金项目:广东省自然科学基金资助项目(No.06029125);广东海洋大学面上项目(No.0812069) |
|
| 摘要点击次数: 2340 |
| 全文下载次数: 2788 |
| 中文摘要: |
| 将裸体方格星虫(Sipunculus nudus)置于0.04%秋水仙素溶液中暂养12h,取其体腔液,用盐度6.50‰的过滤海水低渗45min,1500r/min离心8min,卡诺氏液固定1h,热滴片,5%Giemsa染色,显微观察、摄影,根据Levan等的分类标准进行染色体组型分析。结果表明,裸体方格星虫的染色体基数为x=17,2n=2x=34,染色体组型为2n=2x=26m+8sm,NF=68。除第2、3、4、8对染色体为亚中部着丝粒染色体(sm)外,其余均为中部着丝粒染色体(m)。臂比值变化范围是1.211~2.617。未发现性染色体和随体。 |
| 英文摘要: |
| The samples of Sipunculus nudus were treated with 0.04% colchicine solution for 12 h.The celomic liquid was extracted,treated with 6.5‰ seawater for 45 min,and then centrifuged at 1 500 r/min for 8 min.The sediment was fixed by Carnoy's solution.The suspension was dropped on heated slides.The slides were stained with Giemsa(5%,pH 6.8),rinsed in distilled water and air-dried.Well-spread metaphase chromosomes were examined and photographs were taken with a photomicroscope.The chromosome types were categorized according to Levan's report.It was found that the basic number of chromosomes of S.nudus was x=17,2n=2x=34 and the karyotype formula was 2n=2x=26m+8sm,NF=68.All chromosome pairs were metacentric,except that the chromosome pair numbers 2,3,4,8 were submetacentric.Arm ratio was in range of 1.211-2.617.No sex chromosomes and satellites were observed in the present study. |
| 附件 |
| 查看全文 查看/发表评论 下载PDF阅读器 |
|
|
|
