冯红丽,俞诗源,王昱,贾艳芳,邓海平.2009.金雕肺的组织结构及KGF、c-Fos和Bax的表达.动物学杂志,44(1):33-38. |
金雕肺的组织结构及KGF、c-Fos和Bax的表达 |
Histological Structure and Expression of KGF,c-Fos and Bax in the Lung of Aguila chrysaetos |
投稿时间:2008-08-05 修订日期:2008-11-07 |
DOI: |
中文关键词: 金雕 肺 组织结构 免疫组织化学 角质细胞生长因子 c-Fos蛋白 Bax蛋白 |
英文关键词:Aguila chrysaetos Lung Histological structure Immunohistochemistry KGF c-Fos protein Bax protein |
基金项目:国家自然科学基金项目(No.30370163);甘肃自然科学基金项目(No.ZS031-A25-005-Z);甘肃省创新人才基金项目 |
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中文摘要: |
为了解金雕(Aguila chrysaetos)肺的组织结构特征,观察角质细胞生长因子(KGF)、c-Fos蛋白和Bax蛋白在肺中的表达情况,应用生物显微技术和免疫组织化学方法观察了金雕肺的组织结构,检测KGF、c-Fos和Bax蛋白在肺中的表达。结果表明,金雕的肺由各级支气管及其周围的呼吸毛细管组成,肺房呈辐射状开口于三级支气管周围,呼吸毛细管相互吻合成网状,周围有丰富的毛细血管网。KGF、c-Fos和Bax在三级支气管、肺房和呼吸毛细管的上皮细胞中呈免疫反应阳性,三者的表达强度有差异。KGF、c-Fos和Bax可能发挥不同的功能,它们的协同表达可能调控鸟肺细胞的生长、发育及成熟。 |
英文摘要: |
Histological structure was observed by light microscopy and expression of KGF,c-Fos and Bax was examined by immunohistochemical methods in Aguila chrysaetos lung. Results showed that the lung was consisted of bronchia and many breathing capillaries (minute trachea) around bronchia; pulmonary atrium radially disposed around tertiary bronchia; many breathing capillaries anastomosed reticulation with each other; and rich pulmonary capillaries distributed around minute trachea. KGF,c-Fos and Bax were expressed in the epithelial cells of the tertiary bronchia,pulmonary atrium and breathin capillaries,but their expression intensities were different. KGF,c-Fos and Bax may have different functions,and their cooperative expression may control the growth, development and maturity of cells in bird lung. |
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