The fertilization of Chinese Sturgeon （Acipenser sinensis） milt was assessed by using different concentrations of NaCl,KCl,MgCl₂,CaCl₂ or glucose solution as the insemination-activation medium.The results showed that the fertilization rates of Chinese Sturgeon were all improved when suitable concentrations of NaCl,KCl,MgCl₂,CaCl₂ and glucose solutions were used,and that the fertilization rates reached the highest when the following concentrations of solutions were used: 87.72% in 25 mmol/L NaCl,86.82% in 0.1 mmol/L KCl, 82.24% in 1 mmol/L MgCl₂, 89.76% in 1 mmol/L CaCl₂, and 80.92% in 50 mmol/L glucose.Further increase in the salt or glucose concentration caused gradual decrease in fertilization rate. The results indicate that,as insemination-activation medium of Chinese Sturgeon milt, the most suitable concentration for NaCl, KCl, MgCl₂, CaCl₂ and glucose solution were 25 mmol/L, 0.1 mmol/L, 1 mmol/L, 1 mmol/L, and 50 mmol/L, respectively.