| 魏锁成,张剑.2009.GnRH-A主动免疫家兔的去势效果.动物学杂志,44(1):139-144. |
| GnRH-A主动免疫家兔的去势效果 |
| Castration by GnRH-A Active Immunization in Rabbits |
| 投稿时间：2008-08-25 修订日期：2008-11-05 |
| DOI： |
| 中文关键词: 促性腺激素释放激素类似物(GnRH-A) 抗原 睾酮 免疫去势 家兔 |
| 英文关键词:Gonadotropin releasing hormone analogue (GnRH-A) Antigen Testosterone Immunocastration Rabbit |
| 基金项目:国家民委2002年度重点资助项目(No.MW2002-ZD-010) |
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| 中文摘要: |
| 为了探讨促性腺激素释放激素类似物(gonadotropin releasing hormone analogue,GnRH-A)对动物免疫去势的效果及作用机理。在30只日本大耳白兔(Oryctolagus cuniculus)(EG-Ⅰ与EG-Ⅱ组)的颈背部皮下分2～3点注射1.0ml(100μg/ml)自制的GnRH-A抗原乳剂,EG-Ⅱ组3周后加强免疫一次;测定睾丸长度与重量、体重、血清GnRH抗体效价及睾酮浓度。结果表明,EG-Ⅱ组睾丸长度与EG-Ⅰ组差异极显著(P<0.01);EG-Ⅱ组的GnRH抗体水平明显高于EG-Ⅰ组;EG-Ⅱ组、EG-Ⅰ组与对照组的血清睾酮浓度差异逐渐增加,102d时EG-Ⅱ组睾酮浓度极显著低于对照组(P<0.01),且第28d后EG-Ⅱ组睾酮浓度显著低于EG-Ⅰ组(P<0.05);EG-Ⅱ组体重和日增重最大,显著高于EG-Ⅰ组及对照组(P<0.05)。GnRH-A主动免疫家兔对睾丸发育、血清GnRH抗体效价和睾酮浓度具有显著的影响,加强免疫效果更理想。 |
| 英文摘要: |
| To investigate the efficiency of immunocastration by using gonadotropin releasing hormone analogue (GnRH-A) in rabbits,GnRH-A was combined with bovine serum albumin (BSA) as well as incomplete Freund's adjuvant to make antigen emulsion.A total of 30 rabbits (Oryctolagus cuniculus) were injected with 1.0 ml(100 μg/ml)GnRH-A antigen emulsion at 2 to 3 spots in two groups,EG-Ⅰand EG-Ⅱ groups.The EG-Ⅱgroup was reinjected with the same dosage after 3 weeks.The testis length and weight as well as body weight of the immunized rabbits were measured,and GnRH antibody titer and testosterone concentration were detected by ELISA.There was a significant difference in the testis length between EG-Ⅰand EG-Ⅱ groups(P<0.01); antibody level of EG-Ⅱgroup was obviously higher than that of EG-Ⅰgroup; serum testosterone concentration in both EG-Ⅰgroup and EG-Ⅱgroups differed remarkably from 7 to 16 weeks when compared with the control (P<0.05 ); testosterone concentration in EG-Ⅱgroup was lower than that in control group (CG) at 102 d(P<0.01),and testosterone concentration in EG-Ⅰgroup was also lower at 28 d(P<0.05) after the injecting GnRH-A antigen.Body weight and average daily body weight gain in EG-Ⅱ group were significantly higher than in EG-Ⅰand CG groups (P<0.05).In summary,the testis development,serum GnRH antibody titer and testosterone level in experimental rabbits change obviously after active immunization with GnRH-A and an additional injection strengthenes castration effects. |
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