| 刘秀霞,梁旭方,李观贵,王琳,李光照.2009.鳜鱼CRP cDNA和斜带石斑鱼AAT cDNA的克隆与序列分析.动物学杂志,44(2):84-91. |
| 鳜鱼CRP cDNA和斜带石斑鱼AAT cDNA的克隆与序列分析 |
| Cloning and Sequence Analysis of CRP Gene from Siniperca chuatsi and AAT Gene from Epinephelus coioides |
| 投稿时间：2008-08-19 修订日期：2008-12-30 |
| DOI： |
| 中文关键词: C-反应蛋白 α1-抗胰蛋白酶 基因克隆 序列分析 鳜鱼 斜带石斑鱼 |
| 英文关键词:C-reactive protein α1-antitrypsin Molecular clone Sequence analysis Siniperca chuatsi,/i> Epinephelus coioides |
| 基金项目:国家科技部863项目(No.2007AA09Z437);国家自然科学基金项目(No.30670367);广东省自然科学基金项目(No.031886);广东省科技计划项目(No.2007B020701002) |
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| 中文摘要: |
| 采用RT-PCR及RACE法分别克隆得到鳜鱼(Siniperca chuatsi)C-反应蛋白(C-reactive protein,CRP)cDNA全序列和斜带石斑鱼(Epinephelus coioides)α1-抗胰蛋白酶(α1-antitrypsin,AAT)cDNA全序列。鳜鱼CRP基因cDNA全长为914 bp,其中5′非翻译区(5′-UTR)为49 bp,3′非翻译区(3′-UTR)为199 bp,开放阅读框(ORF)为666 bp,编码222个氨基酸。序列同源性分析发现,推测的鳜鱼CRP氨基酸序列与小鼠(Mus musculus)、人类(Homo sapiens)、大鼠(Rattus norvegicus)、非洲蟾蜍(Xenopus tropicalis)和中国鲎(Tachypleus tridentatus)的CRP氨基酸同源性分别为33.2%、32.4%、31.5%、24.9%和22.4%。斜带石斑鱼肝脏ATT基因cDNA全序列长1 785 bp,其中,5′-UTR为13 bp,3′-UTR为530 bp,ORF为1 242 bp,编码414个氨基酸。序列同源性分析发现,推测的斜带石斑鱼AAT氨基酸序列与斑马鱼(Danio rerio)、非洲爪蟾(X.laevis)、楔齿蜥(Sphenodon punctatus)、大鼠、人类、狒狒(Papio papio)和小鼠的AAT氨基酸序列同源性分别为59.2%、40%、38.6%、38.5%、37.7%、37%和36%。鳜鱼CRP基因和斜带石斑鱼AAT基因cDNA全序列的获得为其疾病相关分子机理研究奠定了基础,对今后进一步进行种苗育苗的研发,并以此为依据提高其人工育苗仔鱼成活率有重要意义。 |
| 英文摘要: |
| Two full-length cDNA sequences encoding C-reactive protein(CRP) gene of Chinese perch(Siniperca chuatsi) and α1-antitrypsin(AAT) gene of orange-spotted grouper(Epinephelus coioides) were isolated by RT-PCR and RACEs methods,respectively.The CRP cDNA is 914 bp in length,and contains a 666 bp open reading frame(ORF) encoding 222 amino acids.The AAT cDNA is 1 785 bp in length,and contains a 1 242 bp ORF encoding 414 amino acids.Sequence analysis reveals that the identity of CRP amino acids between amphibian and mammals is low,ranging at 22.4%-33.2%.The identity of AAT amino acid among fish,mammals and amphibian is also low,ranging at 36%-59.2%.The data are important for future study on molecular structure and function of disease-related genes in aquatic animals,and for improvement of the larvae survival and fry rearing during artificial propagation. |
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