| 董晓敏,周显青,王迎.2009.温度对中华大蟾蜍和中华鳖冬眠前离体红细胞免疫功能及其大小的影响.动物学杂志,44(3):101-107. |
| 温度对中华大蟾蜍和中华鳖冬眠前离体红细胞免疫功能及其大小的影响 |
| Immune Functions and Sizes of Erythrocytes from Pre-hibernal Bufo gargarizans and Trionyx sinensis:in vitro Effects of Temperature |
| 投稿时间：2009-02-05 修订日期：2009-03-26 |
| DOI： |
| 中文关键词: 温度 中华大蟾蜍 中华鳖 红细胞大小 免疫功能 |
| 英文关键词:Temperature Chinese Big Toad(Bufo gargarizans) Soft-shelled Turtle(Trionyx sinensis) Erythrocyte size Immune function |
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| 中文摘要: |
| 为了探讨温度变化对中华大蟾蜍(Bufo gargarizans)和中华鳖(Trionyx sinensis)离体红细胞免疫功能及细胞大小的影响,我们设计了10℃、20℃、30℃、37℃4个温度组,通过红细胞C3b受体花环试验和红细胞免疫复合物花环试验检测红细胞免疫活性,同时测量其红细胞大小。结果表明,温度对中华大蟾蜍离体红细胞C3b受体花环率影响显著,随着温度的升高,中华大蟾蜍离体红细胞C3b受体花环率逐渐降低,在30℃和37℃时,其红细胞C3b受体花环率明显比10℃时低;而温度对其离体红细胞免疫复合物花环率和红细胞大小却没有显著影响。温度对中华鳖离体红细胞C3b受体花环率、免疫复合物花环率及其大小亦没有显著影响。除了10℃时中华鳖离体红细胞免疫复合物花环率与中华大蟾蜍没有明显差别外,其余各温度下中华鳖离体红细胞C3b受体花环率和免疫复合物花环率均明显比中华大蟾蜍的高,而其红细胞的长径和短径却明显比中华大蟾蜍红细胞的小。这说明,温度能明显影响中华大蟾蜍离体红细胞免疫功能,而对中华鳖离体红细胞的免疫活性影响不明显,随着动物进化程度的提高,中华鳖红细胞对温度变化的适应能力和免疫活性比中华大蟾蜍明显增强。 |
| 英文摘要: |
| This study investigated the effects of different temperatures（10℃,20℃,30℃,37℃）on the immune functions and sizes of in vitro erythrocytes from the pre-hibernal Chinese Big Toad（Bufo gargarizans）and the Soft-shelled Turtle（Trionyx sinensis）.The erythrocytic immunocompetence was observed by the tests of erythrocyte-C3b receptor rosette（E-C3bR）and erythrocyte immune complex rosette（E-ICR）,and the long-diameter and short-diameter of erythrocyte were measured.The results showed that,in Chinese Big Toad,the rate of E-C3bR rosette was reduced with temperature increasing from 10℃ to 37℃,significantly lower at 30℃ and 37℃ than at 10℃,indicating that the temperature plays an important role in formation of E-C3bR rosette.However,the rates of E-ICR rosette and erythrocyte sizes had no significant change at different temperatures.Besides,in the Soft-shelled Turtle,the rates of E-C3bR rosette,E-ICR rosette and the erythrocyte size had no significant difference among different temperature groups.The results also demonstrated that the rates of E-C3bR rosette and E-ICR rosette in the Soft-shelled Turtle were higher than those of the Chinese Big Toads at different temperatures,except the rate of E-ICR rosette at 10℃,while the long-diameter and short-diameter of erythrocyte in Soft-shelled Turtle were obviously smaller than those of toads.The results above demonstrate that the temperature significantly affects the immune functions of erythrocyte in Chinese Big Toad,but not in Soft-shelled Turtle.Therefore,with animal evolution,the endurance to the temperature changes and the erythrocyte immunocompetence in Soft-shelled Turtle is higher than that in Chinese Big Toad. |
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