| 金建丽,刘铸,郝爱平,杨春文,金志民.2009.棕背(鼠平)和红背(鼠平)种间区分的分子方法.动物学杂志,44(3):127-131. |
| 棕背(鼠平)和红背(鼠平)种间区分的分子方法 |
| Delimiting the Myodes rufocanus and M. rutilus Species by Molecular Methods |
| 投稿时间：2008-11-19 修订日期：2009-03-29 |
| DOI： |
| 中文关键词: 棕背(鼠平) 红背(鼠平) 种间区分 分子生物学 |
| 英文关键词:Myodes rufocanus M.rutilus Species identification Molecular biology |
| 基金项目:黑龙江省教育厅项目(No.11511420);黑龙江省自然科学基金项目(No.C200626) |
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| 中文摘要: |
| 棕背(鼠平)(Myodes rufocanus)和红背(鼠平)(M.rutilus)的分布有重叠区且外形相似,在特定情况下存在种间区分困难,给两种鼠的数量调查带来不便和误判。本研究通过mtDNA控制区构建系统树、mtDNA控制区电泳和RAPD3种分子生物学方法有效地对棕背(鼠平)和红背(鼠平)已知的8个样本和16个待定样本进行了准确鉴定。其中,mtDNA控制区电泳进行种间区分的方法具有简便、准确而又快捷的优点。 |
| 英文摘要: |
| Myodes rufocanus and M.rutilus have overlapping range and similar morphological characteristics.It is hard to distinguish these two species under some conditions,which brings inconvenience and miscalculation to their number investigations.Eight known samples and sixteen unknown samples of M.rufocanus and M.rutilus are correctly identified by three molecular methods:phylogenetic tree based on sequences of mtDNA control region,electrophoresis of mtDNA control region,and the RAPD).Electrophoresis of mtDNA control region shows the advantages of convenience,accuracy and time-saving. |
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