西北农林科技大学人才基金项目(No.01140502)
- 摘要 | |
- 访问统计 |
- 参考文献 [14] |
- 相似文献 [20] |
- 引证文献 | |
- 文章评论
为探索下丘脑ghrelin mRNA及GnRH mRNA在大鼠(Rattus norregicus)发情期和间情期的表达特点,通过外部观察和阴道涂片相结合的方法确定发情期和间情期,将12只未经产SD雌性大鼠分为2组,即发情期组和间情期组,每组6只。取动物下丘脑,用实时荧光RT-PCR方法检测ghrelin mRNA和GnRH mRNA的表达丰度。结果表明,间情期组大鼠下丘脑ghrelin mRNA的表达丰度显著高于发情期组(P<0.01);间情期组大鼠下丘脑GnRH mRNA的表达丰度显著低于发情期组(P<0.01)。研究发现,下丘脑ghrelin mRNA和GnRH mRNA在发情期与间情期具不同的表达模式,提示ghrelin可能在下丘脑水平上对GnRH mRNA的表达具下调作用。
This study was designed to investigate the expression of ghrelin mRNA in the hypothalamus of rats at the estrus and diestrus.Twelve virginal rats were classified into 2 groups as the estrus(n=6) and diestrus(n=6) which were determined by vaginal smears daily.All animals were killed by decapitation,and samples of hypothalamic tissue were obtained to detect the value of ghrelin and GnRH mRNA by real-time RT-PCR.Results showed that expression of ghrelin mRNA in the hypothalamus changed with the oestrous cycle,higher in the diestrus compared to that in the estrus(P<0.01),while on the contrary GnRH mRNA level was higher in the estrus than that in the diestrus(P<0.01).Results suggest that ghrelin may down-regulate the expression of GnRH mRNA in the hypothalamus.
引用本文
张文龙,童德文,邢福姗.2009.大鼠发情期和间情期下丘脑ghrelin mRNA的表达.动物学杂志,44(4):28-33.
复制文章指标
- 点击次数:2639
- 下载次数: 2856
- HTML阅读次数: 0
历史
- 收稿日期:2008-12-18
- 最后修改日期:2009-04-29
