| 阎雪岚,杨金权,唐文乔,王丽卿,阿达来提·尔迪.2009.基于线粒体Cyt b基因序列变异的克孜河塔里木裂腹鱼和斑重唇鱼遗传多样性.动物学杂志,44(5):8-13. |
| 基于线粒体Cyt b基因序列变异的克孜河塔里木裂腹鱼和斑重唇鱼遗传多样性 |
| Genetic Diversity of Two Cyprinid Fishes:Schizothorax biddulphi and Diptychus maculates in Kezilesu River,Xinjiang Uygur Autonomous Region Based on Cyt b Gene Sequences Variation |
| 投稿时间：2009-01-20 修订日期：2009-06-24 |
| DOI： |
| 中文关键词: 塔里木裂腹鱼 斑重唇鱼 Cytb 遗传多样性 克孜勒苏河 新疆 |
| 英文关键词:Schizothorax biddulphi Diptychus maculates Cytb Genetic diversity Kezilesu River Xinjiang Autonomous Region |
| 基金项目:上海高校水产养殖学E-研究院项目(No.E03009);上海市重点学科建设项目(No.S30701) |
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| 中文摘要: |
| 采用线粒体细胞色素b基因(Cytb)序列,分析了采自新疆克孜河3个群体(斯木哈纳SM、牙师YS、卡拉贝利KL)的塔里木裂腹鱼(Schizothoraxbiddulphi)41尾个体及1个斑重唇鱼(Diptychusmaculates)群体(斯木哈纳)23尾个体的种群遗传多样性和遗传结构。结果显示,塔里木裂腹鱼检测到6个碱基变异位点,定义了4种单倍型,平均单倍型多样性指数及核苷酸多样性指数分别为0.5254和0.00116。分子变异分析(AMOVA)结果提示,塔里木裂腹鱼的遗传变异全部发生于群体内部;群体间Kimura-2-parameter遗传距离、分化指数(Fst<0.08525)和基因流(Nm>3.18)都显示3个群体没有种群分化,属于单一种群。斑重唇鱼检测出7个变异位点,定义了8个单倍型,平均单倍型多样性指数与核苷酸多样性指数分别为0.8301和0.00113。研究表明,克孜河的塔里木裂腹鱼和斑重唇鱼均处于很低的遗传多样性水平,物种维持力较弱。 |
| 英文摘要: |
| The complete mitochondrial Cyt b gene sequences were analyzed to detect the diversity of Schizothorax biddulphi and Diptychus maculates.A total of 41 individuals of S.biddulphi were sampled from 3 localities(Simuhana,Yashi and Kalabeili) and 23 individuals of D.maculates were sampled from 1 locality(Simuhana) of Kezilesu River,West Xinjiang autonomous region.In S.biddulphi,6 variable sites were detected and 4 haplotypes were defined,which has low haplotype diversity(0.525 4) and low nucleotide diversity(0.001 16).Analysis of molecular variance(AMOVA) indicated that all of the genetic variations observed were found within three populations.The average Kimura 2-parameter distances among the three populations of S.biddulphi,the population differentiation values(Fst<0.085 25) and the level of gene flow(Nm>3.18) all showed that there was no genetic differentiation among these three populations,indicating that the three populations belong to a single population.In D.maculates,7 variable sites were detected and 8 haplotypes were defined,and the haplotype diversity and nucleotide diversity were 0.830 1 and 0.001 13,respectively.Both S.biddulphi and D.maculates have low genetic diversity,indicating their weak viability to maintain population. |
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