Taking consideration of the fact that microsatellite primers can be amplified among different species in the same genus,family and order,we used 70 Rat(Rattus norvegicus) and Mouse(Mus musculus) microsatellite primers to amplify their counterparts in a close species-the Chinese White-Bellied Rat(Niviventer confucianus) through PCR amplification,polyacrylamide gel electrophoresis and silver staining technique.A total of 40 loci could be amplified in agarose gel electrophoresis,but after polyacrylamide gel electrophoresis,only 21 loci could be stably amplified,among which 15 loci showed hybridism and 13 loci showed polymorphism.Mg~ 2+ concentration in PCR amplification was at 1.5 and 2.0 mmol/L,and the annealing temperature ranged from 50℃ to 60℃. The existence of stutter bands in partial amplified products did not affect the selection of purpose bands. Overall,it is feasible to amplify the Chinese white-bellied microsatellite primers by using their rat and mouse counterparts.