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胡先成,李佳坤,赵云龙.2020.河川沙塘鳢胚胎、仔鱼发育过程中 蛋白质含量及氨基酸库的变化.动物学杂志,55(6):776-783.
河川沙塘鳢胚胎、仔鱼发育过程中 蛋白质含量及氨基酸库的变化
Changes in the Protein Content and Amino Acid Pool during Embryonic and Larval Development of Odontobutis potamophila
投稿时间:2020-04-29  修订日期:2020-10-28
DOI:10.13859/j.cjz.202006011
中文关键词:  河川沙塘鳢  胚胎发育  仔鱼发育  蛋白质  氨基酸库
英文关键词:Odontobutis potamophila  Embryonic development  Larval development  Protein  Amino acid pool
基金项目:重庆市科委自然科学基金项目(No. 7877),重庆市教委科学技术研究项目(No. KJ090827),重庆市科技创新能力建设项目(No. 2010CA1010)
作者单位E-mail
胡先成 重庆师范大学重庆市动物生物学重点实验室 重庆 401331 754578103@qq.com 
李佳坤 重庆师范大学重庆市动物生物学重点实验室 重庆 401331 294398783@qq.com 
赵云龙 华东师范大学生命科学学院 上海 200241 yl zhao@bio.edu.cn 
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中文摘要:
      采用生物化学方法测定和分析了河川沙塘鳢(Odontobutis potamophila)胚胎、仔鱼发育过程中蛋白质含量及氨基酸的组成和含量。结果显示,从受精卵开始到孵出后8 d饥饿仔鱼的整个胚胎和仔鱼发育过程中,其总蛋白和总氨基酸含量均呈下降趋势;总的必需氨基酸含量和总的非必需氨基酸含量也一样都呈现出下降趋势;平均含量最高的氨基酸是亮氨酸Leu、赖氨酸Lys、谷氨酸Glu和天冬氨酸Asp。与总氨基酸库相反,游离氨基酸库却呈现出随着发育的进行而不断增长的趋势,这种增长趋势也反映在游离的必需氨基酸和游离的非必需氨基酸含量的变化上,并且平均含量最高的游离氨基酸是赖氨酸Lys、亮氨酸Leu、脯氨酸Pro和谷氨酸Glu。整个发育过程中,游离氨基酸的含量仅占总氨基酸库的很小一部分(在受精卵,仅占0.16%)。由于总蛋白和总氨基酸含量在发育过程中均呈下降趋势,而游离氨基酸含量呈增长趋势,由此可知,在整个胚胎发生过程中,其卵黄蛋白的水解速度大于胚体同化和异化作用对氨基酸的消耗速度。
英文摘要:
      The Odontobutis potamophila belongs to Perciformes, Odontobutidae. It is an important freshwater commercial fish distributing in the Yangtze River and its tributaries, China. There is no report on the nutritional requirements and metabolic characteristics of the early development of O. potamophila. The content of proteins as well as the composition and content of amino acids during the embryonic and larval development of O. potamophila were analyzed. The fertilized eggs (Ⅰ), embryos at cleavage stage (Ⅱ), blastula stage (Ⅲ), gastrula stage (Ⅳ), lens formation stage (Ⅴ), eye pigmentation stage (Ⅵ), prehatching stage (Ⅶ), and larvae of 1 day after hatching (Ⅷ), 8 days after hatching (Ⅸ) were collected for biochemical analysis. The results indicated that a significant reduction was detected in the total protein content during the embryonic and larval development (P < 0.05, Fig. 1). Eighteen kinds of total amino acids (TAA) and nineteen kinds of free amino acids (FAA) were detected. The TAA content decreased during the embryonic and larval development (Table 1). Overall, the changes in the content of total essential amino acids (TEAA) and the content of total non-essential amino acids (TNEAA) reflected that of the TAA content. Qualitatively, the predominant amino acids were leucine, lysine, glutamic acid and aspartic acid during the embryonic and larval development. In contrast, the FAA pool increased with development (Table 2), the changes were reflected in both the free essential amino acids (FEAA) and the free non-essential amino acids (FNEAA), and the predominant free amino acids were lysine, leucine, proline and glutamic acid. Throughout development, the FAA accounted for only a small proportion (0.16% in fertilised eggs) of the TAA content. Because the egg protein content and the TAA content declined with development, the FAA pool increased during development, and it was concluded that the rate of breakdown of yolk protein was higher than the anabolic and catabolic processes during embryogenesis.
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