| 秦粉菊,袁红霞,聂继华,童建.2010.纳米硒通过抗氧化应激调节大脑NO含量改善睡眠剥夺小鼠认知功能.动物学杂志,45(1):42-49. |
| 纳米硒通过抗氧化应激调节大脑NO含量改善睡眠剥夺小鼠认知功能 |
| Cognition Performance in Sleep-Deprived Mice is Improved by Cerebral NO Content Adjustment via Anti-Oxidation of Nano-Selenium |
| 投稿时间：2009-05-09 修订日期：2009-11-12 |
| DOI： |
| 中文关键词: 纳米硒 睡眠剥夺 认知 谷胱甘肽过氧化物酶 一氧化氮 丙二醛 |
| 英文关键词:Nano-Selenium Sleep deprivation Cognition GSH-Px NO MDA |
| 基金项目:苏州科技学院科研基金项目(No.Z974) |
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| 中文摘要: |
| 研究了纳米硒对睡眠剥夺(SD)小鼠(Mus musculus)认知功能的影响,并探讨其作用机制。将120只雄性昆明小鼠随机分成两批,第一批24只分为3组:对照组(NC)、亚硒酸钠组(SE)和纳米硒组(NS),分别给予硒浓度为4μg/ml的亚硒酸钠和纳米硒溶液每只0.5ml/d,NC组给等体积蒸馏水,连续30d,第31天测定SE和NS两组小鼠的血硒及全血GSH-Px活性,评价两种硒源的生物利用性;第二批96只分为4组:对照组(N-SeC),纳米硒低、中、高剂量组(L、M、H),L、M和H组分别给予硒浓度为2μg/ml、4μg/ml、8μg/ml的纳米硒溶液每只0.5ml/d,N-SeC组给予同体积蒸馏水,连续30d。第二批小鼠每组又各自分为4小组:SD对照组(SDC)及SD18h、SD36h、SD54h组,采用单平台水环境法(SPM)制作小鼠SD模型。在SD后,N-SeC、L、M和H组利用Y-型迷宫试验测定认知能力,同时测定小鼠大脑GSH-Px、NO、MDA含量。结果表明,纳米硒对GSH-Px活性的提高优于传统硒源亚硒酸钠,但血硒无显著差异;与SDC组比较,SD降低了小鼠的认知能力及大脑GSH-Px活性,提高了NO和MDA含量;与N-SeC比较,纳米硒使SD小鼠的认知功能得到改善,大脑GSH-Px活性提高,MDA和NO含量下降。上述结果表明,纳米硒能够改善SD小鼠的认知功能,这可能与其提高大脑GSH-Px活性并降低了自由基对大脑神经的损害有关。 |
| 英文摘要: |
| To study the effects and mechanisms of Nano-Se on cognition performance after sleep deprivation(SD) in mice.A total of 120 male mice were randomly divided into 2 batches.The first batch of 24 mice was randomly divided into 3 groups:control(NC),Na2SeO3(SE) and Nano-Se(NS).These mice were administered respectively with SE and NS(1 μg/d,2 μg/d,4 μg/d) by intra-gastric injection for 30 days,then their Se content and GSH-Px activity in blood were measured; The second batch of 96 mice were randomly divided into 4 large groups:control(N-SeC) and low,middle,high Nano-Se groups(L,M,H).Nano-Se solutions(2 μg/ml,4 μg/ml,8 μg/ml)were administered(0.5 ml/d) to mice of L,M and H groups respectively by intra-gastric injection for 30 days,and the control group was administered with distilled water.Each large group was divided into 4 groups:SD control(SDC),SD18h,SD36h and SD54h.SD model was induced in mice by single platform technique.In the SD mice,the cognitive ability of the mice were tested by Y-maze.Further,brain GSH-Px,NO and MDA levels were measured.Blood GSH-Px activity was enhanced by Nano-Se compared to SE group,but the blood Se content was not different.SD decreased cognitive ability and GSH-Px activity,but increased NO and MDA levels.Compared to N-SeC,Nano-Se improved cognitive ability,increased GSH-Px activities,while decreased NO levels in M and H groups.MDA reduction was found in H group only.Our data suggest that Nano-Se could improve cognitive ability of mice,the mechanism of which might involve the increased GSH-Px activity and decreased free radical content. |
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