国家自然科学基金项目(No.30970454)
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为了探讨有机氯农药硫丹对小鼠(Mus musculus)红细胞免疫功能的影响,设计了体内、外两组实验。体内实验:将40只小鼠随机分成4组,灌胃硫丹的量依次为:0、0.4、1.6、6.4mg/(kg·d)。灌胃25d后,取血测定红细胞的免疫功能。体外实验:将9只小鼠的红细胞分别与不同浓度的硫丹在体外培养,实验设空白对照组、溶剂丙酮组和4个不同浓度硫丹组,其6组实验所用硫丹的量依次为:0、0、5、10、20、40μg/ml。体外培养2h后,测定红细胞免疫粘附能力。结果表明,在活体实验中,随着硫丹浓度的增加,小鼠红细胞C3b受体花环率(ratio of C3b rosetting,C3bRR)明显下降,依硫丹灌胃浓度由低到高,其C3bRR依次为7.78%、6.80%、4.96%、4.33%;而循环免疫复合物花环率(ratio of immune complexes rosetting,ICRR)随着硫丹浓度升高而升高,分别为6.69%、6.31%、7.86%、9.42%。红细胞促NK细胞活性的功能在各组间没有显著差异。硫丹6.4mg/(kg·d)组小鼠红细胞对T淋巴细胞免疫粘附促进能力较其他3组明显降低。血浆中红细胞天然免疫促进因子活性在1.6mg/(kg·d)组和6.4mg/(kg·d)组较0mg/(kg·d)组明显降低。与溶剂丙酮组相比,血浆中红细胞天然免疫抑制因子活性在0.4mg/(kg·d)组明显下降,而在6.4mg/(kg·d)组却明显升高。离体红细胞经硫丹处理后其C3bRR显著降低,4个硫丹处理组依其浓度由低到高,C3bRR依次为:6.14%、5.56%、5.06%、4.44%;而ICRR却显著升高,分别为6.69%、6.31%、7.86%、9.42%。这表明,硫丹能抑制小鼠红细胞免疫粘附能力和红细胞对T淋巴细胞的正向调节功能,降低血浆中红细胞天然免疫促进因子活性,而对抑制因子活性影响比较复杂,低剂量时起抑制作用,而高剂量时能促进其活性。
To investigate the effects of organ chlorine pesticide—endosulfan on erythrocyte immune functions in mice,in vivo and in vitro experiments were designed.A total of 40 mice were randomly divided into 4 groups in in vivo experiment.They were gavaged with endosulfan at levels of 0,0.4,1.6,and 6.4 mg/(kg·d)for 25 days,respectively.Blood was then collected to determine the immune functions of erythrocytes.In In vitro experiment,the erythrocytes from 9 mice were incubated with different concentrations of endosulfan:0(control),0(acetone,dissolvent),5,10,20,and 40 μg/ml,respectively.The immune adherent capacity of the erythrocyte was determined after culturing for 2 hours in vitro.In vivo experiment showed that the ratio of erythrocyte-C3b rosetting(C3bRR) significantly decreased,while the ratio of immune complexes rosetting(ICRR) increased with the increase of endosulfan dosage.There was no significant difference among the 4 groups in the activity of regulating NK cells by erythrocytes.The activity of regulating T cell adhering function by erythrocytes in 6.4 mg/(kg·d)group was significantly lower compared with other three groups.The activity of erythrocyte natural adhering enhancing factor in plasma remarkably reduced in 1.6 mg/(kg·d)group and 6.4 mg/(kg·d)group compared with that of the control group.The activity of erythrocyte natural adhering inhibitory factor decreased in 0.4 mg/(kg·d) group,but increased in 6.4 mg/(kg·d) group.In vitro,the C3bRR of erythrocytes declined,while the ICRR ascended with the increase of endosulfan concentration.These results suggest that endosulfan inhibits the erythrocyte immune adherent function and the positive regulating function of T cells by erythrocytes,and decreases the immune-enhancing activity of erythrocyte.The results also show that the effects of endosulfan on inhibitory factor activity are complicated:it inhibits the activity of inhibitory factor at the low dosage,while improves the activity of inhibitory factor at the high dosage.
引用本文
张濛,周显青.2010.硫丹对小鼠红细胞免疫功能的影响.动物学杂志,45(1):50-58.
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历史
- 收稿日期:2009-06-29
- 最后修改日期:2009-11-18
