From July 2008 to February 2009,we conducted a survey on the bats species diversity in 47 caves at 14 townships in eastern of Songtao County,Guizhou Province. We found it showed 14 species belonging to 3 families,one of them need to identify further. Three species, Pipistrellus javanicus, P. paterculus, and Rhinolophus monoceros are the new records to Guizhou Province,and R. monoceros was found first time in the mainland of China. We also collected information on the hibernation behavior and habitat selection of bats,as well as the threats to their habitat. The hibernation behavior was obvious differences between different species of bat,and the species variation between caves might be indicated by the habitat type difference out of the cave.