Abstract:Primers were designed according to the GenBank sequence of FSHβ gene of the homologous species of Cricetulus barabensis. Partial sequence of exon 3 in FSHβ gene was cloned by PCR method from random samples of C. barabensis in Yinan,Linqu and Qufu,Shandong Province. It is 775 bp and its accession number in GenBank is GQ456067. The homologies of nucleotide and amino acid are 80%-96% and 79%-100% respectively when compared with the same region sequence of Homo sapiens,Rattus norvegicus,Mus musculus, Pan troglodytes,and Ovis aries. The result of the phylogenetic evolution analysis by FSHβ sequence is in consistent with the relative species relationship,and therefore the sequence of FSHβ gene could be used as an ideal marker to study the species relationships or hereditary distance.
