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黑线仓鼠FSHβ基因外显子3部分序列的克隆与分析
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基金项目:

国家973计划项目(No.2007CB109104);国家自然科学基金项目(No.30470247,30670335)


Cloning and Sequencing of Partial Exon 3 of FSHβ Gene in Cricetulus barabensis
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    摘要:

    根据GenBank数据库中黑线仓鼠(Cricetulus barabensis)同源物种的FSHβ基因设计引物,利用PCR法在山东省沂南、临朐和曲阜3个地理种群随机选取的黑线仓鼠个体中克隆出FSHβ基因的部分外显子3,序列长度为775bp(GenBank登录号:GQ456067)。该序列与其他物种相应区域的同源性比较结果显示:黑线仓鼠与人(Homo sapiens)、大鼠(Rattus norvegicus)、小鼠(Mus musculus)、黑猩猩(Pantroglodytes)、羊(Ovis aries)等物种核苷酸序列的同源性为80%～96%,氨基酸序列的同源性为79%～100%。系统进化分析结果与物种亲缘关系的远近一致,说明该研究所得到的FSHβ基因序列可作为研究物种亲缘关系或遗传距离的理想标记。

    Abstract:

    Primers were designed according to the GenBank sequence of FSHβ gene of the homologous species of Cricetulus barabensis. Partial sequence of exon 3 in FSHβ gene was cloned by PCR method from random samples of C. barabensis in Yinan,Linqu and Qufu,Shandong Province. It is 775 bp and its accession number in GenBank is GQ456067. The homologies of nucleotide and amino acid are 80%-96% and 79%-100% respectively when compared with the same region sequence of Homo sapiens,Rattus norvegicus,Mus musculus, Pan troglodytes,and Ovis aries. The result of the phylogenetic evolution analysis by FSHβ sequence is in consistent with the relative species relationship,and therefore the sequence of FSHβ gene could be used as an ideal marker to study the species relationships or hereditary distance.

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靳鹏,张菲菲,解学辉,王洪伟,徐来祥.2010.黑线仓鼠FSHβ基因外显子3部分序列的克隆与分析.动物学杂志,45(2):60-68.

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  • 收稿日期:2009-08-19
  • 最后修改日期:2010-01-04
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