| 王龙海,陈贵海,江伟,张琼,王超.2010.昆明小鼠学习记忆能力的年龄效应及其与海马突触囊泡蛋白的相关性.动物学杂志,45(3):30-36. |
| 昆明小鼠学习记忆能力的年龄效应及其与海马突触囊泡蛋白的相关性 |
| Correlation of Learning and Memory Capability with Age and the Syt Ⅰ Amount in Hippocampus in Kunming Mice |
| 投稿时间：2009-12-10 修订日期：2010-03-01 |
| DOI： |
| 中文关键词: 衰老 小鼠 学习和记忆 海马突触囊泡蛋白 组织微阵列 |
| 英文关键词:Aging Mouse Learning and memory Synaptotagmin 1 Tissue microarray |
| 基金项目:国家自然科学基金项目(No.30872730);安徽省高校省级自然科学研究重大项目(No.ZD2008007.1) |
|
| 摘要点击次数: 2203 |
| 全文下载次数: 2316 |
| 中文摘要: |
| 为了探讨昆明小鼠(Musmusculus)年龄相关性空间学习记忆能力改变及其与海马突触前囊泡蛋白1(synaptotagmin1,SytⅠ)含量之间的关系。选取3个年龄段的昆明小鼠,①青年组:6月龄,28只;②中年组:11月龄,22只;③老年组:22月龄,17只。利用六臂辐射状水迷宫(RAWM)任务检测其空间学习记忆能力;制作组织微阵列,采用免疫组化技术检测SytⅠ在海马中的的表达;采用方差分析方法对六臂辐射状水迷宫实验参数和SytⅠ的相对含量进行统计学分析,使用Spearman秩相关检测这二者之间的相关性。结果发现,老年组昆明小鼠在学习期及记忆期的平均错误数及潜伏期均高于中年和青年鼠(P<0.05),中年昆明小鼠与青年鼠之间的差异不显著(P>0.05);老年组昆明小鼠在海马CA3区及齿状回(dentategyrus,DG区)中SytⅠ的相对含量显著高于中年鼠和青年鼠(P<0.05);昆明小鼠海马CA3、DG区SytⅠ的相对含量与学习期和记忆期的错误数及潜伏期均成正相关(P<0.05)。由此推断,昆明小鼠可出现年龄相关性空间学习记忆能力降低,其海马CA3和DG的SytⅠ相对含量出现年龄相关性升高,海马SytⅠ升高可能与昆明小鼠年龄相关性空间学习记忆能力减退有关。 |
| 英文摘要: |
| In order to explore the correlation of spatial learning and memory functions with aging and SytⅠlevel in hippocampus,three groups of Kunming mice (Mus musculus)at different ages (22 months,11 months and 6 months,respectively) were used. The ability of spatial learning and memory was evaluated by six-arm radial water maze ( RAWM ),the expression of Syt Ⅰ was detected by the immunohistochemistry method ( S-P method),and the data of RAWM and Syt Ⅰ relative content were analysed. It is showed that the average number of errors and latency during learning and memory phase was higher in the 22-month-old group than that in the middle-aged or young group (P < 0. 05),and that there was no significant difference between the middle- aged mice and young mice ( P > 0. 05 ). Meantime,the relative Syt Ⅰ amount in the DG and CA3 ofhippocampus in the 22-month-old mice was significantly higher than that in the middle-aged or young mice ( P < 0. 05). The positive correlation was confirmed between the relative level of Syt I in the DG and CA3 of hippocampus and the number of errors and latency during learning and memory phase (P < 0. 05). Therefore, the results indicate that age-related impairment of spatial learning and memory exists,and the amount of SytⅠ in hippocampus significantly increases with aging in Kunming mice, and that the amount of Syt Ⅰ in hippocampus may be involved in the age-related spatial cognitive impairment. |
| 附件 |
| 查看全文 查看/发表评论 下载PDF阅读器 |
|
|
|
