国家自然科学基金项目(No.30370163);甘肃省创新人才基金项目(ZS006-016)
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应用免疫组织化学方法检测了Bax蛋白、TGF-β(TGF-β2、TGF-β3)和Ghrelin在饥饿后长耳鸮(Asiootus)胃及小肠中的表达,利用体视学方法对其表达强度进行半定量分析。结果表明,胃及小肠呈Bax、TGF-β2、TGF-β3和Ghrelin免疫反应阳性。Bax免疫反应阳性物质主要分布在胃黏膜、肠黏膜、肠腺的单层柱状上皮细胞,腺胃深腺单层立方上皮细胞及胃黏膜固有层;TGF-β2、TGF-β3免疫反应阳性物质主要分布在胃黏膜、肠黏膜及肠腺的单层柱状上皮细胞,腺胃深腺单层立方上皮细胞及肠黏膜固有层;Ghrelin免疫反应阳性细胞分布在腺胃深腺,十二指肠、空肠、回肠的黏膜、肠腺的单层柱状上皮细胞及黏膜固有层。Bax蛋白阳性表达部位的面密度值在腺胃有最大值,从腺胃到十二指肠面密度值下降,十二指肠到回肠逐渐上升;TGF-β2和TGF-β3阳性表达部位的面密度值在十二指肠达到峰值,TGF-β2从十二指肠到回肠阳性表达部位的面密度值呈递减趋势,TGF-β3从腺胃到十二指肠面密度值逐渐增大,之后下降,到回肠回升;Ghrelin阳性表达部位的面密度值从腺胃到回肠逐渐减小。Bax、TGF-β2、TGF-β3及Ghrelin在饥饿后长耳鸮胃和小肠各段均有不同程度的表达,它们可能参与长耳鸮胃肠功能的调节。
The expression of Bax,TGF-β2,TGF-β3 and Ghrelin in the stomach and small intestine of Asio otus after starvation was investigated by immunohistochemical staining,and their expression intensity was analyzed semiquantitatively by a stereological method. The stomach and small intestine showed immunoreactivity of Bax, TGF-β2 ,TGF-β3 and Ghrelin. Bax immunopositive cells were mainly distributed in the single-layered columnar epithelial cells of stomach mucosa,intestinal mucosa,intestinal glands and the single-layered cuboidal epithelial cells in deep glands of the glandular stomach. TGF-β2 and TGF-β3 immunopositive cells were mainly localized in the single-layered columnar epithelial cells of the mucosa of the glandular stomach,simple tubular glands of muscular stomach,intestinal mucosa,intestinal glands as well as the single-layered cuboidal epithelial cells in deep glands of the glandular stomach and lamina propria in small intestine. Ghrelin immunopositive cells weremainly distributed in the single-layered columnar epithelial cells of the intestinal mucosa,intestinal glands as well as the single-layered cuboidal epithelial cells in deep glands of the glandular stomach and lamina propia of small intestine mucosa. The expression of Bax showed the maximum in the glandular stomach,decreased progressively from glandular stomach to duodenum,and then increased from duodenum to ileum. The expression of TGF-β2 and TGF-β3 showed the maximum in the duodenum. The expression of TGF-β2 decreased progressively from duodenum to ileum,while the expression of TGF-β3 increased from glandular stomach to duodenum,decreased progressively after duodenum,and then increased in ileum. Ghrelin immunopositive cell density gradually decreased from the glandular stomach to the ileum. Bax,TGF-β2 ,TGF-β3 and Ghrelin may participate functional regulation of stomach and small intestine.
引用本文
王悦,俞诗源,高先军,郭婷婷.2010. Bax、TGF-β和Ghrelin在饥饿后长耳鸮胃及小肠中的免疫组织化学.动物学杂志,45(3):127-132.
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- 收稿日期:2009-11-19
- 最后修改日期:2010-02-23
