| 解美娜,李锋杰,丁保清,赵惠.2010.哺乳期母鼠灌服双酚A对雄性后代生殖发育的影响及其机制.动物学杂志,45(4):31-38. |
| 哺乳期母鼠灌服双酚A对雄性后代生殖发育的影响及其机制 |
| Effects of Bisphenol A Exposed to Lactating Mice on the Testis Development of Their Male Offspring |
| 投稿时间：2009-12-28 修订日期：2010-04-23 |
| DOI： |
| 中文关键词: 双酚A 哺乳期 睾丸 ERα PCNA |
| 英文关键词:Bisphenol A Lactation Testis ERα PCNA |
| 基金项目:山东省高等学校科技计划项目(No.J08LG77);潍坊医学院青年教师启动基金项目(No.KQ07004) |
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| 中文摘要: |
| 母鼠(Mus musculus)分娩后第2天起每天灌服双酚A(BPA,剂量5、50、100mg/kg),仔鼠通过乳汁暴露于BPA,一直持续到第22天断奶,研究BPA对雄性仔鼠成年后精子生成、睾丸结构与睾丸内雌激素受体(ER)α和增殖细胞核抗原(PCNA)表达的影响及雌激素、抗氧化酶水平变化。结果发现,BPA处理组血清谷胱甘肽-过氧化物酶(GSH-Px)和总超氧化物歧化酶(SOD)活力显著降低,雌激素水平升高。所有剂量组睾丸发育受到抑制,生精细胞排列紊乱,精子畸形率显著升高,5和100mg/kg剂量组附睾尾精子数降低。免疫组织化学结果显示,不同曲细精管间ERα表达呈现出两种模式,一种是仅在支持细胞核内表达,另一种是在支持细胞和精母细胞核内均表达。PCNA表达与ERα表达有一定相关性,后一种ERα表达模式的曲细精管内PCNA阳性细胞显著增多。哺乳期接触BPA雄鼠睾丸内ERα单细胞平均表达强度升高,初级精母细胞PCNA表达量升高。 |
| 英文摘要: |
| Bisphenol A (BPA,at dose of 5,50,or 100 mg /kg) was given daily by gavage to female mice (Mus musculus) during lactation,and thus the pre-weaning baby mice were exposed to BPA from maternal milk. The effects of BPA on testis development and the expression of estrogen receptor (ER) α and proliferating cell nuclear antigen (PCNA) in testes were studied. In addition,the serum level of estrogen and some antioxidant enzymes were detected. Results showed that serum concentration of estrogen in mature male mice was higher than that in the vehicle-treated group. The exposed mice had a significant decrease in the activities of glutathione peroxidase (GSH-Px) and the total superoxide dismutase (SOD). Morphological studies revealed that testis development was inhibited by BPA with germ cells in disordered arrangement. BPA reduced the number of caudal epididymal sperm and increased the number of abnormal sperm significantly. Immunohistochemistry analysis revealed that there were two different patterns of ERα expression,presence only in the nuclei of Sertoli cells or in the nuclei of spermatocytes except for Sertoli cells. PCNA-positive cells were increased in the latter ERα expression pattern. The average integrated optical density (IOD) of ERα-positive single germ cell in BPA-treated groups was significantly higher than that in the vehicle-treated group. The expression of PCNA in primary spermatocytes was also increased in BPA-treated groups. |
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