| 付博,房庆昌,任亮,汪亮,孙洪涛,别墅,马红,刘娣.2010.培养基中能量底物对猪胚胎发育的影响.动物学杂志,45(4):164-170. |
| 培养基中能量底物对猪胚胎发育的影响 |
| Effect of Energy Substrates on the Development of Porcine Embryos |
| 投稿时间：2009-12-22 修订日期：2010-03-15 |
| DOI： |
| 中文关键词: 猪 能量底物 孤雌胚 移核胚 |
| 英文关键词:Pig Energy substrate Parthenogenetic embryos Nuclear transfer embryos |
| 基金项目:国家“十一五”科技支撑计划重点项目(No.2008BADB2B02);转基因生物新品种培育重大专项(No.2008ZX08006-003) |
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| 中文摘要: |
| 旨在探讨丙酮酸和乳酸对猪(Susscrofa)胚胎早期发育的影响,将NCSU-23培养基中的5.56mmol/L葡萄糖替换为0.2mmol/L丙酮酸、5.7mmol/L乳酸,并将此培养基命名为mNCSU-23。根据实验设计,孤雌胚及核移植胚转移到mNCSU-23或NCSU-23中培养。激活第2天统计孤雌胚及核移植胚中的5～8细胞胚胎数。激活第6天统计孤雌胚及核移植胚囊胚形成率及囊胚细胞数。实验结果表明,mNCSU/NCSU处理组的5～8细胞胚胎数及囊胚数显著高于对照组(P<0.05);单纯使用mNCSU培养猪胚胎时,囊胚率最低,发育结果最差(P<0.05)。本研究证实,在体外培养前两天,用乳酸和丙酮酸代替培养基中的葡萄糖对胚胎发育有利。 |
| 英文摘要: |
| This study aimed to investigate the effects of pyruvate and lactic acid on early development of porcine embryos. The NCSU-23 culture medium was modified by replacing 5.56 mmol /L glucose with 0.2 mmol /L pyruvate and 5.7 mmol /L lactic acid,and the modified medium was called mNCSU-23. Parthenogenetic embryos and nuclear transfer embryos were transferred into either NCSU-23 or mNCSU-23 medium. Embryo development to 5-8 cells was observed 2 days after egg activation,and blastocyst rate and the number of nuclei in the blastocyst were determined on day 6. A higher proportion of the embryos reached 5-8 cells at 48 h and blastocysts on day 6 in mNCSU /NCSU treatment (first 48 h in mNCSU-23 medium and thereafter in NCSU-23 medium) group when compared with the control (NCSU /NCSU,first 48 h in NCSU-23 medium and thereafter also in NCSU-23 medium) group (P < 0.05). The rate of blastocyst formation in mNCSU-23 /mNCSU-23 (first 48 h in mNCSU-23 medium and thereafter also in mNCSU-23 medium) group was the lowest (P < 0.05). Our results have demonstrated that replacing glucose with pyruvate and lactic acid during the first 48 h of IVC may be beneficial to the development of porcine embryos. |
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