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付博,房庆昌,任亮,汪亮,孙洪涛,别墅,马红,刘娣.2010.培养基中能量底物对猪胚胎发育的影响.动物学杂志,45(4):164-170.
培养基中能量底物对猪胚胎发育的影响
Effect of Energy Substrates on the Development of Porcine Embryos
投稿时间:2009-12-22  修订日期:2010-03-15
DOI:
中文关键词:    能量底物  孤雌胚  移核胚
英文关键词:Pig  Energy substrate  Parthenogenetic embryos  Nuclear transfer embryos
基金项目:国家“十一五”科技支撑计划重点项目(No.2008BADB2B02);转基因生物新品种培育重大专项(No.2008ZX08006-003)
作者单位E-mail
付博 黑龙江省农业科学院 哈尔滨 150086 liudi1963@163.com 
房庆昌 东北农业大学生命科学学院 哈尔滨 150030  
任亮 东北农业大学生命科学学院 哈尔滨 150030  
汪亮 东北农业大学生命科学学院 哈尔滨 150030  
孙洪涛 东北农业大学生命科学学院 哈尔滨 150030  
别墅 东北农业大学生命科学学院 哈尔滨 150030  
马红 黑龙江省农业科学院 哈尔滨 150086
东北农业大学生命科学学院 哈尔滨 150030 
 
刘娣 黑龙江省农业科学院 哈尔滨 150086
东北农业大学生命科学学院 哈尔滨 150030 
 
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中文摘要:
      旨在探讨丙酮酸和乳酸对猪(Susscrofa)胚胎早期发育的影响,将NCSU-23培养基中的5.56mmol/L葡萄糖替换为0.2mmol/L丙酮酸、5.7mmol/L乳酸,并将此培养基命名为mNCSU-23。根据实验设计,孤雌胚及核移植胚转移到mNCSU-23或NCSU-23中培养。激活第2天统计孤雌胚及核移植胚中的5~8细胞胚胎数。激活第6天统计孤雌胚及核移植胚囊胚形成率及囊胚细胞数。实验结果表明,mNCSU/NCSU处理组的5~8细胞胚胎数及囊胚数显著高于对照组(P<0.05);单纯使用mNCSU培养猪胚胎时,囊胚率最低,发育结果最差(P<0.05)。本研究证实,在体外培养前两天,用乳酸和丙酮酸代替培养基中的葡萄糖对胚胎发育有利。
英文摘要:
      This study aimed to investigate the effects of pyruvate and lactic acid on early development of porcine embryos. The NCSU-23 culture medium was modified by replacing 5.56 mmol /L glucose with 0.2 mmol /L pyruvate and 5.7 mmol /L lactic acid,and the modified medium was called mNCSU-23. Parthenogenetic embryos and nuclear transfer embryos were transferred into either NCSU-23 or mNCSU-23 medium. Embryo development to 5-8 cells was observed 2 days after egg activation,and blastocyst rate and the number of nuclei in the blastocyst were determined on day 6. A higher proportion of the embryos reached 5-8 cells at 48 h and blastocysts on day 6 in mNCSU /NCSU treatment (first 48 h in mNCSU-23 medium and thereafter in NCSU-23 medium) group when compared with the control (NCSU /NCSU,first 48 h in NCSU-23 medium and thereafter also in NCSU-23 medium) group (P < 0.05). The rate of blastocyst formation in mNCSU-23 /mNCSU-23 (first 48 h in mNCSU-23 medium and thereafter also in mNCSU-23 medium) group was the lowest (P < 0.05). Our results have demonstrated that replacing glucose with pyruvate and lactic acid during the first 48 h of IVC may be beneficial to the development of porcine embryos.
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