| 邵爱华,杜建,陈葵,盛烨,郑峰丰,史全良,沈卫德,朱江.2010.暗纹东方鲀线粒体基因组核苷酸全序列测定与分析.动物学杂志,45(5):18-28. |
| 暗纹东方鲀线粒体基因组核苷酸全序列测定与分析 |
| Sequence and Analysis of the Complete Mitochondrial Genome of Takifugu fasciatus |
| 投稿时间：2010-03-17 修订日期：2010-07-02 |
| DOI： |
| 中文关键词: 暗纹东方鲀 线粒体基因组 全序列分析 |
| 英文关键词:Takifugu fasciatus Mitochondrial genome Sequence analysis |
| 基金项目:农业部行业标准资助项目(No.2002-04084);苏州科技学院2005年度科研基金项目(No.2005-Z862) |
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| 中文摘要: |
| 以暗纹东方鲀(Takifugu fasciatus)肝的线粒体DNA为模板,参照红鳍东方鲀(T.rubripes)等近源鱼类的线粒体基因组DNA序列,设计合成14对特异引物,进行PCR扩增并测序,首次获得了暗纹东方鲀线粒体基因组全序列。结果表明,暗纹东方鲀线粒体基因组序列全长16 444 bp(GenBank登录号为GQ409967),A+T含量为55.8%,其mtDNA结构与其他脊椎动物相似,由22个tRNA基因、2个rRNA基因、13个蛋白质编码基因和1段819 bp非编码的控制区(D-loop)所组成。蛋白质基因除COⅠ和ND6的起始密码子为GTG、CCT以外,均为典型的起始密码子ATG。ND1、ATPase8、COⅢ、ND4L、ND5、Cyt b使用典型的终止密码子TAA,其他的使用不完全终止密码子。除ND6和tRNAGln、tRNAAla、tRNAAsn、tRNACys、tRNATyr、tRNASer、tRNAGlu、tRNAPro在L-链上编码之外,其余基因均在H-链编码。基因排列顺序与已测定的鲀类一致,这显示了鲀类线粒体基因排列顺序上的保守性。tRNA基因核苷酸长度为64～73nt,预测了22个tRNA基因的二级结构,均呈较为典型的三叶草状。基于19种鲀类mtDNA全序列构建的进化树表明,暗纹东方鲀与红鳍东方鲀、中华东方鲀(T.chinensis)聚成一个姊妹群。结果还支持东方鲀属鱼类为一单系类群。 |
| 英文摘要: |
| The nucleotide sequence of the complete mitochondrial DNA(mtDNA) genome was determined in Takifugu fasciatus.DNA sequencing was based on PCR fragments from 14 primer pairs that were designed on the basis of mtDNA sequences of other related Takifugu species.The entire mitochondrial genome of T.fasciatus(GenBank accession no.GQ409967) was composed of 16 444 base pairs(bp) in length with 55.8% A + T content,which coded for 22 tRNAs,2rRNAs,13 protein-coding genes,as well as a control region(D-loop) of 819 bp,as was characteristic for mitochondrial genomes of other metazoans.All protein-coding genes of the T.fasciatus mitochondrial genome started with a typical ATG codon,except for COⅠ and ND6 with GTGand CTT.The usual TAA termination codons were found for ND1,ATPase8,COⅢ,ND4L,ND5 and Cyt b,however,the other 7 protein-coding genes had an incomplete termination codons(T or TA).It was comfirmed that the gene arrangement of T.fasciatus could be used to identify other Tetrodontiformes fishes.Transfer RNA(tRNA) genes ranged in length from 64 to 73 nt,and their planar structures showed characteristic clover leaf.Construction of phylogenetic trees based on the entire mitochondrial genome sequence of 19 Tetrodontiformes species constructed by NJ and MP method has suggested that the T.fasciatus has closer relationship to the T.rubripes and T.chinensis,and that they constitute a sister group.The results also suggest that the genus Takifugu(Tetrodontiformes,Tetraodontidae) can be treated as a monophyletic group. |
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