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乌梢蛇精巢间质细胞微细结构与血清 睾酮浓度的相关性
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The Correlation between Fine Structure of Leydig Cells and Serum Testosterone in Chinese Rat Snake Zaocys dhumnades
Author:
  • CHANG Xiao-Juan

    CHANG Xiao-Juan

    College of Life Sciences, Shaanxi Normal University; Key Laboratory of Ministry of Education for Medicinal Resources and Natural Pharmaceutical Chemistry; National Engineering Laboratory for Resource Development of Endangered Crude Drugs in Northwest of China, Xian 710062,China
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  • LIANG Gang

    LIANG Gang

    College of Life Sciences, Shaanxi Normal University; Key Laboratory of Ministry of Education for Medicinal Resources and Natural Pharmaceutical Chemistry; National Engineering Laboratory for Resource Development of Endangered Crude Drugs in Northwest of China, Xian 710062,China
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  • WANG Qiong-Xia

    WANG Qiong-Xia

    College of Life Sciences, Shaanxi Normal University; Key Laboratory of Ministry of Education for Medicinal Resources and Natural Pharmaceutical Chemistry; National Engineering Laboratory for Resource Development of Endangered Crude Drugs in Northwest of China, Xian 710062,China
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  • 摘要
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  • 参考文献 [28]
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    摘要:

    用光镜及透射电镜观察了乌梢蛇(Zaocys dhumnades)精巢间质细胞的显微与超微结构,并利用放射免疫测定法测定了血清中睾酮浓度。结果表明,在一个年生殖周期中,乌梢蛇间质组织所占区域相对大小、间质细胞数量和显微结构均存在较明显的变化;5月份的间质细胞具有发达的管状嵴线粒体、丰富的滑面内质网、大量的脂滴等合成和分泌类固醇激素的超微结构特征;血清中睾酮浓度变化明显,5月份出现主峰,9月份出现次峰。间质细胞的显微和超微结构特征及其变化与血清中睾酮浓度变化具有相关性,表明乌梢蛇间质细胞能够合成与分泌睾酮等性类固醇激素。

    Abstract:

    The microstructure and ultrastructure of Leydig cells in Chinese Rat Snake Zaocys dhumnade were examined by light microscopy and transmission electron microscopy,and the concentration of serum testosterone was measured by radioimmunoassay (RIA).During a reproductive cycle,the region of Leydig tissue,the quantity of Leydig cells and the microstructure changed very evidently.In the Leydig cells in May,there were developed mitochondria with tubular cristae,abundant smooth endoplasmic reticulum and numerous lipid droplets,suggesting that Leydig cells have the ultrastructure characteristic of synthesizing and secreting steroid hormone.The concentration of serum testosterone also changed evidently,and its major peak appeared in May,and the minor peak appeared in September.The microstructure and ultrastructure of Leydig cells were positively correlated with the concentration of serum testosterone.Our results suggest that Leydig cells in Chinese Rat Snake can synthesize and secrete testosterone.

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    [28] Wibbels T,Owens D W,Licht P,et al.Seasonal changes in serum gonadotropins and gonadal steroids associated with migration,mating,and nesting in the loggerhead sea turtle (Caretta caretta).General and Comparative Endocrinology,1990,79(1): 154-164. 图 版 说 明 1.5月精巢光镜图,示间质组织的大小与间质细胞的形态,×400; 2.9月精巢光镜图,示间质细胞分布均匀与大的间质细胞,×400; 3.9月精巢光镜图,示间质细胞成群分布,×400; 4.5月间质细胞电镜图,示脂滴、滑面内质网和线粒体,×15 000; 5.5月间质细胞电镜图,示发达的管状嵴线粒体,×10 000; 6.5月间质细胞电镜图,示较多的糖原颗粒,×10 000; 7.5月间质细胞电镜图,示丰富的脂滴,×5 000; 8.8月间质细胞电镜图,示细胞核、溶酶体、线粒体,×10 000; 9.9月间质细胞电镜图,示脂滴及靠近核膜的异染色质,×12 000; 10.9月间质组织电镜图,示辐射状排列的新增生间质细胞、胞质中的糖原颗粒,×5 000; 11.1月间质组织电镜图,示间质细胞核呈梭形、间质组织中的纤维状物质,×10 000; 12.1月间质细胞电镜图,示小的脂滴和线粒体,×10 000。 Explanation of Plate 1.Light microscope figure of testis in May showing the size of Leydig tissue and morphology of Leydig cells,×400; 2.Light microscope figure of testis in September showing evenly distributed Leydig cells and some big Leydig cells,×400; 3.Light microscope figure of testis in September showing crowds of Leydig cells,×400; 4.Electron microscope figure of a Leydig cell in May showing lipid droplets,smooth endoplasmic reticulum and mitochondria,×15 000; 5.Electron microscope figure of a Leydig cell in May showing mitochondria with tubular cristae,×10 000; 6.Electron microscope figure of a Leydig cell in May showing many giycogenosomes,×10 000; 7.Electron microscope figure of a Leydig cell in May showing abundant lipid droplets,×5 000; 8.Electron microscope figure of a Leydig cell in August showing nucleus,lysosome and mitochondria,×10 000; 9.Electron microscope figure of a Leydig cell in September showing lipid droplets and heterochromatin close to nuclear membrane,×12 000; 10.Electron microscope figure of Leydig cells in September showing giycogenosome and new radialized Leydig cells,×5 000; 11.Electron microscope figure of a Leydig cell in January showing fusiform nucleus and fibrous matter in Leydig tissue,×10 000; 12.Electron microscope figure of a Leydig cell in January showing few lipid droplets and mitochondria,×10 000.Ch:异染色质; F:纤维状物质; G:糖元颗粒; L:脂滴; Lc:间质细胞; Ly:溶酶体; Mi:线粒体; N:细胞核; Ser:滑面内质网。 Ch: Heterochromatin; F: Fibrous matter; G: Giycogenosome; L: Lipid droplet; Lc: Leydig cell; Ly: Lysosome; Mi: Mitochondria; N: Nucleus; Ser: Smooth endoplasmic reticulum. 常小娟等:乌梢蛇精巢间质细胞微细结构与血清睾酮浓度的相关性 图版ⅠCHANG Xiao-Juan et al.: The Correlation between Fine Structure of Leydig Cell and Serum Testosterone in Chinese Rat Snake Zaocys dhumnades PlateⅠ
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常小娟,梁刚,王琼霞.2011.乌梢蛇精巢间质细胞微细结构与血清 睾酮浓度的相关性.动物学杂志,46(1):29-35.

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  • 收稿日期:2010-08-02
  • 最后修改日期:2010-11-09
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