| 薛德明.2011.盘基网柄菌野生型KAx-3发育过程中自发荧光的观察.动物学杂志,46(1):56-61. |
| 盘基网柄菌野生型KAx-3发育过程中自发荧光的观察 |
| Autofluorescence of KAx-3 Cells during Development in Wild Type Dictyostelium discoideum |
| 投稿时间：2010-07-02 修订日期：2010-11-07 |
| DOI： |
| 中文关键词: 盘基网柄菌野生型KAx-3 发育 自发荧光 |
| 英文关键词:Dictyostelium discoideum wild type strain KAx-3 Development Autofluorescence |
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| 中文摘要: |
| 社会性变形虫盘基网柄菌(Dictyostelium discoideum)是研究发育和致病机理的重要模式生物之一。发育过程中自发荧光的研究有利于流式细胞仪检测时确定基础荧光阈值、细胞分选和判定荧光标记实验的准确性。本文用荧光显微镜对盘基网柄菌野生型KAx-3不同发育阶段在4种不同波长的激发光(蓝光、绿光、黄光和紫外线)照射下的自发荧光现象进行了观察。结果表明,在蓝光激发下,子实体阶段孢子囊自发荧光最强,单细胞期最弱;在绿光和黄光激发下,蛞蝓体阶段无自发荧光产生;在紫外线激发下,单细胞期最弱,自发荧光强弱会发生变化;蓝光激发会产生绿色荧光,绿光和黄光激发产生红色荧光,紫外线激发产生蓝色荧光。以上结果说明盘基网柄菌自发荧光光谱具有多样性。 |
| 英文摘要: |
| The social amoeba Dictyostelium discoideum is increasingly being used as a simple model for the investigation of problems that are relevant to development and pathogenesis.The investigation of autofluorescence during development is helpful to identifying basic fluorescent threshold,accuracy of experiment marked with fluorescent dye,and cellular sorting with flow cytometry.The autofluorescence of KAx-3 cells at different developmental phases was observed with fluorescent microscope in wild type D.discoideum.The results showed that the autofluorescence of spore capsule at fruiting body was strongest,while the autofluorescence was weakest at unicellular phase under excitement of wide blue (abbr: WB).There was no autofluorescence at slug under excitement of wide green (abbr: WG) and wide interference yellow (abbr: WIY).The autofluorescence at unicellular phase was weakest under excitement of wide ultraviolet ray (abbr: WU).The autofluorescent intensity was changed during development.Green,red,and blue colors of autofluorescences were separately excited by WB,WG and WIY,and WU.The results indicate the diversity autofluorescence spectrum of KAx-3 cells in wild type D.discoideum. |
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