| 黄映萍,王莹,苗素英.2011.粤东海域口虾蛄遗传多样性.动物学杂志,46(2):82-89. |
| 粤东海域口虾蛄遗传多样性 |
| Genetic Diversity Analysis of Mantis Shrimps (Oratosquilla oratoria) from the Eastern Coast of Guangdong Province |
| 投稿时间：2010-10-19 修订日期：2010-12-28 |
| DOI： |
| 中文关键词: 口虾蛄 遗传多样性 线粒体DNA COⅠ基因 |
| 英文关键词:Oratosquilla oratoria Genetic diversity Mitochondrial DNA Cytochrome oxidase subunit Ⅰ |
| 基金项目:国家自然科学基金项目(No.30671612); |
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| 中文摘要: |
| 采用线粒体COⅠ基因序列对粤东汕尾和深圳2个海域口虾蛄(Oratosquilla oratoria)的遗传多样性进行了分析。研究表明,所分析的口虾蛄mtDNA COⅠ基因(592 bp)共检测到21个变异位点,占总位点的3.55%。转换和颠换位点数分别为18和3个,碱基替换的饱和性分析表明,口虾蛄COⅠ基因碱基替换未达到饱和,COⅠ基因适合作为分析口虾蛄遗传多样性研究的分子标记。所检测的个体一共有15个单倍型,单倍型多样性为0.971,核苷酸多样性为0.007 55。单倍型之间的遗传距离在0.047 6~0.428 6之间,平均遗传距离为0.214 1。两群体间遗传分化系数FST为0.036,群体单倍型之间的UPGAM聚类以及NETWORK亲缘关系网状图并未显示明显的地理聚类结果。粤东海域口虾蛄总体遗传多样性水平较高,遗传分化小。 |
| 英文摘要: |
| As a routine and important polymorphic marker, mitochondrial cytochrome oxidase subunit Ⅰ (COⅠ) gene was used to analyze the genetic diversity for two populations of Oratosquilla oratoria from Shenzhen and Shanwei coast of Guangdong Province. The result revealed that 21 of the 592 bp (3.55%) nucleotides were variable, among which 18 and 3 sites were identified as transition and transversion respectively. The substitution saturation analysis indicated that COⅠ gene was not satured in O.oratoria and could be used as an effective molecular tool for population genetic studies. Totally, 15 haplotypes were detected. The nucleotide diversity and haplotype diversity were 0.007 55 and 0.971 respectively. The fixation index (FST) was 0.036 between two populations, suggesting little differentiation between them. There was no geographic clustering of haplotypes in the UPGAM tree and the network. Genetic distances among haplotypes ranged from 0.047 6 to 0.428 6, with an average value of 0.214 1. In summary, a high level of genetic diversity and little genetic differentiation are observed in O.oratoria from the eastern coast of Guangdong province. |
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