欢迎访问动物学杂志

首页关于本刊期刊订阅编委会作者指南过刊浏览

郝莹莹,施志仪,李文娟,靳雨丽.2011.维生素D₃对三角帆蚌外套膜细胞内Ca²⁺浓度的影响.动物学杂志,46(3):98-103.

维生素D₃对三角帆蚌外套膜细胞内Ca²⁺浓度的影响

Effect of VD₃ on Intracellular Calcium Ion Concentration in Mantle Cells of Hyriopsis cumingii:An in Vitro Study

投稿时间：2010-11-30 修订日期：2011-03-04

DOI：

中文关键词: 三角帆蚌外套膜细胞Ca²⁺ 浓度维生素D₃(VD₃) 激光共聚焦扫描显微镜

英文关键词:Hyriopsis cumingii Intracellular Ca²⁺concentration of mantle VD₃ Confocal laser scanning microscope

基金项目:上海市科委基础重大项目(No.06aj14003),上海市重点学科水生生物学建设项目(No.S30701),上海市博士后科研资助计划面上项目(No.09R21413200);

作者	单位	E-mail
郝莹莹	上海海洋大学农业部水产种质资源与养殖生态重点开放实验室上海 201306
施志仪	上海海洋大学农业部水产种质资源与养殖生态重点开放实验室上海 201306	zyshi@shou.edu.cn
李文娟	上海海洋大学农业部水产种质资源与养殖生态重点开放实验室上海 201306
靳雨丽	上海海洋大学农业部水产种质资源与养殖生态重点开放实验室上海 201306

摘要点击次数: 2754

全文下载次数: 2375

中文摘要:

采用胰酶消化法获得三角帆蚌( Hyriopsis cumingii )外套膜细胞,用Fluo-3/AM 荧光标记技术和激光共聚焦扫描显微镜(confocal laser scanning microscope,CLSM)检测外套膜内外表皮细胞在静息状态下细胞的游离Ca²⁺浓度及不同浓度维生素D₃(VD₃)孵育后的外套膜细胞的Ca²⁺的动态情况。结果表明,未添加VD₃(1.25 mmol/L Ca²⁺)时,外套膜外表皮细胞的Ca²⁺荧光强度显著高于内表皮细胞内的Ca²⁺( P <0.05)。在添加不同浓度VD₃后,内表皮细胞与外表皮细胞内Ca²⁺浓度变化趋势一致,表现为细胞内Ca²⁺沉积量的变化是随着VD₃浓度的增大而增大,其中,对照组与添加50 IU/L组差异不显著( P >0.05);当添加浓度为100 IU/L时,与对照组和50 IU/L组差异显著( P <0.05);当添加组浓度达到500 IU/L时,与前3组有显著差异( P <0.05);当添加的浓度增大到1 000 IU/L后与500 IU/L组差异不显著( P >0.05),但与其他组均存在显著差异( P <0.05)。本实验为外套膜细胞的钙代谢机制研究提供了实验依据。

英文摘要:

The mantle cells from Hyriopsis cumingii were obtained by using trypsin digestion. The Ca²⁺ concentrations in inner mantle cells and outer mantle cells under the resting condition and the dynamics of Ca²⁺concentration in the mantle cells incubated with various concentrations of VD₃ were measured by Fluo-3/AM fluorescent labeling and CLSM. The results showed that the average Ca²⁺ fluorescence intensity of outer mantle cells was significantly higher than that of inner mantle cells ( P <0.05). After adding different concentrations of VD_3,the average Ca²⁺ fluorescence intensity was enhanced with the increased concentration of VD₃ in culture medium, and inner mantle cells and outer mantle cells showed the same change. The difference between the control and 50 IU/L VD₃ groups, or between 500 IU/L and 1 000 IU/L groups was not significant ( P >0.05), but the difference between the control and another three groups (100 IU/L, 500 IU/L and 1 000 IU/L), or between the 50 IU/L group and another three groups(100 IU/L, 500 IU/L and 1 000 IU/L) was significant ( P <0.05). The present research may provide theoretical basis for promoting the pearl industry.

附件

查看全文查看/发表评论下载PDF阅读器