| 王悦,邓海平,俞诗源.2011.X射线对仔鼠皮肤组织结构及c-Fos、KGF表达的影响.动物学杂志,46(3):123-130. |
| X射线对仔鼠皮肤组织结构及c-Fos、KGF表达的影响 |
| Effect of X-ray on Histological Structure as well as c-Fos and KGF Expression in Developing Mouse Skin |
| 投稿时间：2010-10-15 修订日期：2011-03-17 |
| DOI： |
| 中文关键词: X射线 仔鼠 皮肤 组织结构 c-Fos KGF |
| 英文关键词:X-ray Develoing Mouse Skin Histological structure C-Fos KGF |
| 基金项目:国家自然科学基金项目 (No.30370163),甘肃省创新人才基金项目; |
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| 中文摘要: |
| 应用生物显微技术和免疫组织化学方法,研究了不同剂量X射线(0.0、3.5、6.5 Gy)辐射对发育期(1、5、10和20 d)昆明小鼠( Mus musculus )仔鼠皮肤组织结构和c-Fos、KGF表达的影响,探讨了c-Fos、KGF的生物活性作用及调控意义,并利用IPP专业图像分析软件对其表达强度进行定量分析。结果表明,辐射后1 d,皮肤组织结构无明显变化;辐射后5 d,表皮、真皮及皮下组织厚度减小,毛的数量急剧减少,皮肤组织纤维化严重;辐射后10 d,3.5 Gy组纤维化现象减轻,真皮致密结缔组织和皮下组织也已基本恢复正常状态,6.5 Gy组仔鼠皮肤组织结构恢复缓慢;辐射后20 d,3.5 Gy组仔鼠皮肤结构基本恢复正常,6.5 Gy组仔鼠全部死亡。图像分析显示,c-Fos和KGF蛋白在各组仔鼠皮肤组织中均有不同程度的表达,辐射组c-Fos和KGF的阳性表达较对照组明显增强,随着辐射剂量的增大,c-Fos、KGF蛋白的表达强度呈递增趋势;随着时间的推移,辐射组c-Fos的阳性表达强度逐渐下降,KGF的阳性表达强度呈现波浪形变化。结果提示,大剂量电离辐射导致仔鼠皮肤组织严重损伤,细胞大量凋亡和坏死,辐射后一段时间,由于机体对损伤的自我适应和调节,皮肤组织损伤情况逐渐减弱最终消失。 |
| 英文摘要: |
| The effect of different dosages (0.0,3.5,6.5 Gy) of X-ray on histological structure and expression of c-Fos and KGF in skin of developing Mouse( Mus musculus )(1 d,5 d,10 d,20 d) were investigated. Results showed that skin structure was not obviously changed after 1 d irradiation; thickness of epidermis,dermis,hypodermis and number of hair decreased,and serious fibrosis appeared in skin of developing Mouse after 5 d irradiation;fibrosis reduced,dermis dense connective tissue and hypodermis recovered gradually after 10 d irradiation with 3.5 Gy of X-ray,while histological structure recovered slowly after irradiation with 6.5 Gy of X-ray. The histological structure of skin recovered after 20 d irradiation with 3.5 Gy of X-ray,while all individuals died after 20 d irradiation with 6.5 Gy of X-ray. The skin of developing Mouse exhibited c-Fos and KGF immunoactivity,and the expression of c-Fos and KGF in radiation groups was obviously increased than that of control groups. The greater dosage was used,the stronger expression of c-Fos and KGF was observed. The expression of c-Fos decreased gradually,while the expression of KGF displayed wave pattern after irradiation with 3.5 or 6.5 Gy of X-ray. There was a trend that histological structure and expression of c-Fos and KGF recovered slowly to the normal state after irradiation. The results indicate that high dosages irradiation causes serious damage to the skin of developing Mouse as well as cell apoptosis and necrosis. After irradiation,the body possess self-healing function,and the number of cells in skin increases obviously, and histological structure of skin recovers gradually. |
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